Figures (5)  Tables (2)
    • Figure 1. 

      (a) Sequence distribution of DETs among Biological Processes (BP) GO terms. (b) Sequence distribution of DETs among Cellular Components (CC) GO terms. (c) Sequence distribution of DETs among Molecular Functions(MF) GO terms.

    • Figure 2. 

      Venn diagram of pair-wise comparisons of DETs unique to timepoints and most enriched GO 210 terms among unique DETs at each time point.

    • Figure 3. 

      GO sequence distribution chart separated by biological process (BP), molecular function (MF) and cellular component (CC).

    • Figure 4. 

      Visualization of differentially expressed genes involved in biotic stress pathways in response to P. obducens (a) 0 h (hours post-inoculation), (b) 12 h, (c) 24 h, (d) 48 h, (e) 120 h and (f) 240 h. Color gradient represents log2 fold ratios with red representing upregulation and green representing downregulation in treatments over mock roots. Each box represents one transcript.

    • Figure 5. 

      Heat map of putative plant defense DETs of I. walleriana during infection by P. obducens.

    • Sample IDRaw reads (PE)Clean reads
      0H_Control_R123,520,86818,287,675
      0H_Control_R227,793,05824,193,950
      12H_Control_R124,320,45220,299,572
      12H_Control_R219,672,88815,319,055
      24H_Control_R124,320,45221,024,513
      24H_Control_R219,672,88813,922,270
      48H_Control_R124,162,18217,057,095
      48H_Control_R217,226,07219,581,661
      120H_Control_R120,526,63423,639,470
      120H_Control_R223,321,21618,259,179
      240H_Control_R127,654,81014,085,034
      240H_Control_R221,765,00421,712,471
      0H_Infected_R137,971,71229,005,241
      0H_Infected_R237,778,30229,248,606
      12H_Infected_R133,006,92024,968,942
      12H_Infected_R231,223,42624,440,783
      24H_Infected_R134,379,44226,500,567
      24H_Infected_R236,662,54427,860,148
      48H_Infected_R131,490,40223,140,744
      48H_Infected_R233,238,08424,795,526
      120H_Infected_R137,946,60229,078,825
      120H_Infected_R234,596,69225,346,127
      240H_Infected_R133,746,88823,867,301
      240H_Infected_R233,041,34824,552,405
      Total528,964,774

      Table 1. 

      Statistical summary of RNA sequencing data.

    • Comparison
      (infected vs
      control)
      Total
      DETs
      Upregulated
      DETs
      Downregulated
      DETs
      Unique to
      timepoint
      DETs
      0 h4,5492,4702,068298
      12 h4,0982,1321,966140
      24 h4,3692,2352,133201
      48 h3,6192,0061,613112
      120 h3,6072,0661,54197
      240 h4,4912,6721,819502

      Table 2. 

      Pairwise comparisons between infected vs control samples at all six time points.