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Figure 1. 

Protein mass distribution of Lilium davidii var. unicolor peptides.

Figure 2. 

Gene ontology (GO) analysis of all proteins during the bulblets development indentified by iTRAQ analysis. The left y-axis indicates the percentage of a specific category of genes existed in the main category, whereas the right y-axis indicates the number of a specific category of genes existed in main category. The classification of these proteins in different categories are shown based on (a) biological process, (b) molecular function and (c) cellular component.

Figure 3. 

COG classification of peptides.

Figure 4. 

GO enrichment diagrams of down-regulated and down-regulated DEPs in three comparisons. (a) Comparison of 15 d vs 0 d, (b) Comparison of 30 d vs 15 d, (c) Comparison of 30 d vs 0 d.

Figure 5. 

GO pathway enrichment bubble charts. (a), (b) and (c) represent the GO pathway name for DEPs in 15 d vs 0 d, 30 d vs 15 d and 30 d vs 0 d, respectively. The size of the bubble indicates the number of DEPs annotated on a GO term. The color represents the enriched p-value.

Figure 6. 

Statistical chart of up-regulated and down-regulated proteins enriched pathway results in the three comparisons. (a), (b) and (c) represent the comparison of 15 d vs 0 d, 30 d vs 15 d and 30 d vs 0 d, respectively.

Figure 7. 

NCBI ORF finder result and gene expression changes of LdGASA at different development stages of lily bulblets by real-time PCR. The values are the mean ± SD of three independent biological replicates, and lowercase and uppercase letters indicate statistically significant differences at p < 0.05 and p < 0.01 respectively as determined by Duncan's test.

Figure 8. 

Overexpression of LdGASA in transgenic Arabidopsis thaliana. (a) The LdGASA CDS was driven by the enhanced CaMV35S promoter. PTF/PTR used for identification of LdGASA in plants. (b) Quantitative RT-PCR analysis of LdGASA transcript levels in corresponding transgenic Arabidopsis leaves. The expression levels were indicated as that of WT as control. The values are the mean ± SD of three independent biological replicates normalized against the reference gene Actin. Lowercase and uppercase letters indicate statistically significant differences at p < 0.05 and p < 0.01 respectively as determined by Duncan's test. (c) The whole plants at 3 weeks old. Scales bars 1 cm. (d) The height and fresh weight of seedling at 3 weeks old. Plant height and fresh weight were determined based on six replicates for each genotype. Vertical bars represent mean ± SD. Lowercase and uppercase letters indicate statistically significant differences at p < 0.05 and p < 0.01 respectively as determined by Duncan's test.