Figures (4)  Tables (3)

    • Figure 1. 

      Fusarium sp. (UST-UVG10) isolated from the leaves of U. valderramensis. (a), (b) Observed and reverse view of the colony on PDA plates after 14 d of incubation. (c), (d) Micrographs; macro- and microconidia.

    • Figure 2. 

      The phylogenetic tree of isolated UST-UVG10, based on ITS region amplification, reveals genus-level identification due to its species complexity. Numbers in the node indicate the bootstrap values.

    • Figure 3. 

      LC-HRESIMS chromatograms of the EtOAc extracts of Fusarium sp. (UST-UVG10). (a) ZM ½, (b) YM 6.3, (c) Q6 ½.

    • Figure 4. 

      Structures of the major antibiotic compounds fusaric acid (1) and beauvericin (2) identified from Fusarium sp. (UST-UVG10) extracts.

    • TaxaStrain no.GenBank accession numbers (ITS)Ref.
      Fusarium dlaminiiNRRL 13164TNR 182425[24]
      Fusarium dlaminiiNRRL 13164U34572[25]
      Fusarium acutatumNRRL 13308U34573[25]
      Fusarium acutatumCBS 402.97MH862652[26]
      Fusarium acutatumCBS 739.97MH862669[26]
      Fusarium acutatumCBS 402.97TNR_111142[27]
      Fusarium phyllophilumNRRL 13617TNR_182426[25]
      Fusarium phyllophilumNRRL 13617U34574[25]
      Fusarium hostaeNRRL 29888TNR 171109[28]
      Fusarium fujikuroiCBS 221.76AB725607[29]
      Fusarium fujikuroiCBS 221.76TNR_111889[27]
      Fusarium fujikuroiCBS 221.76MW827608[30]
      Fusarium hechienseLC13644TNR_182846[31]
      Fusarium hechienseLC13644MW016454[31]
      Fusarium elaeagniLC13627TNR_182845[31]
      Fusarium elaeagniLC13627MW016426[31]
      Fusarium sp.NRRL28852AF158304[32]
      Fusarium sp.NRRL26793AF158312[32]
      Fusarium nygamaiCBS 749.97MH862671[26]
      Fusarium nygamaiNRRL 13448TNR 130698[25]
      Fusarium nygamaiCBS 749.97MT740750[33]*
      Fusarium aquaticumLC7502TNR 182844[31]
      Fusarium aquaticumLC7502MW016408[31]
      Fusarium panlongenseLC13656TNR 182847[31]
      Fusarium panlongenseLC13656MW016470[31]
      T Type specimens. * The species Fusarium nygamai (CBS 749.97) with the accession number - MT740750, was not yet published. www.ncbi.nlm.nih.gov/nuccore/2100342893.

      Table 1. 

      Reference taxa used in this study with their strain number and GenBank accession numbers.

    • ExtractZone of inhibition (mm)
      YM 6.3Culture media6.56
      Mycelia6
      Q6 ½Culture media6
      Mycelia6
      ZM ½Culture media13.85
      Mycelia8.11
      Streptomycin31.84

      Table 2. 

      Antibacterial activity of the fungal crude extracts against Staphylococcus aureus.

    • Extract/CompoundMIC vs S. aureus (μg/mL)MABA MIC vs M. tb H37Rv (μg/mL)
      YM 6.3−62
      Q6 ½−>64
      ZM ½−>64
      Fusaric acid (1)1.3052.4
      Streptomycin1.30−
      Isoniazid−0.25
      Rifampin−0.12

      Table 3. 

      Minimum Inhibitory Concentrations (MICs) of the fungal extracts against Staphylococcus aureus and Mycobacterium tuberculosis H37Rv.