Back Article

Figure 1. 

Phylogenetic tree of the LRX proteins from Arabidopsis, rice, and seven cucurbit species. Red star, purple star, light green star, deep red star, sky blue star, deep blue star, deep brown star, deep green star and light brown star represent Arabidopsis thaliana (A. thaliana), rice (O. sativa), cucumber (C. sativus ), melon (C. melon), watermelon (C. lanatus), wax gourd (B. hispida), pumpkin (C. maxima), bottle gourd (L. siceraria) and moschata pumpkin (C. moschata), respectively.

Figure 2. 

Phylogenetic clustering, conserved motifs, conserved domains and gene structures of seven cucurbits LRX genes. Left one: The phylogenetic tree of LRX genes. Light green and brownish red represent LRX and PEX subfamilies, respectively. Left two: the conserved motif of LRX genes, different colors represent different motifs; right one: LRX gene structure analysis, green and blue represent the UTR region and the CDS region, respectively; right two: the conserved domain of LRX genes, different colors represent different domains.

Figure 3. 

Synteny analysis of LRX genes. (a) Synteny analysis of LRX genes between cucumber and other six cucurbits. (b) Synteny analysis of LRX genes in cucumber, Arabidopsis thaliana (A. thaliana) thaliana and rice (O. sativa). (c) Colinearity analysis of CsLRX genes in cucumber species. The red and blue lines represent gene pairs with collinearity.

Figure 4. 

Cis-acting elements in the promotors of LRX genes in seven cucurbit species. The colors indicate the different cis-elements numbers. Values indicate the statistical number of cis elements.

Figure 5. 

The expression pattern of LRX genes in different Cucurbitaceae plant tissues. (a) The expression of public RNA-seq data of LRX genes in different tissues of Cucurbitaceae; (b) qRT-PCR analysis of CsLRX genes in different tissues of cucumber. Values are means ± SD of three biological replicates.

Figure 6. 

Expression analysis of CsLRX genes in cucumber. (a) Cucumber roots were treated with 100 mM NaCl and 10% PEG6000, and water was used as a control (mock). (b-c) The CsLRX genes in cucumber roots treated with 100 mM NaCl and 10 % PEG6000 for different time was analyzed by qRT-PCR. Values are means ± SD of three biological replicates. Significant differences between 0 h and other time points are indicated by asterisks (ns: no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, Student’s t test). (d) Subcellular localization of CsLRX1 and CsLRX3. The empty vector was used as a control. These indicated structures were transiently expressed in N. benthamiana leaves. Bar, 50 μm.

Figure 7. 

Interaction network prediction of CsLRX protein in cucumber.