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Figure 1. 

Chromosomal localization of PosHSF genes in P. suffruticosa.

Figure 2. 

Phylogenetic relationships of HSF proteins across P. suffruticosa, A. thaliana, and O. sativa.

Figure 3. 

Characterization of PosHSF genes. (a) Conserved protein structural domains: the green color indicates the most conserved DBD structural domain in the HSF protein. (b) Amino acid motifs in PosHSF: motifs 1−10 in PosHSF are numbered and analyzed. (c) Gene structure of PosHSF, overview of the gene structure in PosHSF genes. (d) Multiple sequence alignment of the DBD structural domain in PosHSF proteins.

Figure 4. 

Analysis of promoter cis-acting elements of PosHSF. (a) Heat map and categorization of PosHSF promoter cis-acting elements. (b) Distribution numbers of the three categories of promoter cis-acting elements in the PosHSF gene.

Figure 5. 

(a) Synteny analysis of HSF genes in P. suffruticosa, A. thaliana, and Z. mays. (b) Synteny analysis of HSF genes in P. suffruticosa, P. ludlowii, and V. vinifera.

Figure 6. 

Expression profiles of tissue-specific PosHSF genes in different tissues.

Figure 7. 

Analysis of heat tolerance transcriptome data in P. suffruticosa. (a) WGCNA heatmap of module-trait correlations. (b) K-means clustering of gene expression in the blue module. (c) Heatmap of PosHSF expression under high-temperature treatment. Red boxes indicate the modules of focus.

Figure 8. 

Expression levels of 15 HSF members of P. suffruticosa in RNA-seq and qRT-PCR. The red line represents the results of RNA-seq, and the black line represents qRT-PCR.