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    • Figure 1. 

      Flesh color of 'TianYu' and 'TianJu' at different stages of fertility. (a) Represents the color of 'TianYu' flesh at 15 d post-pollination; (b) depicts the color of 'TianYu' flesh at 30 d post-pollination; (c) illustrates the color of 'TianJu' flesh at 15 d post-pollination; (d) portrays the color of 'TianJu' flesh at 30 d post-pollination.

    • Figure 2. 

      Contents of various types of carotenoids in the pulp of 'TianYu' and 'TianJu' fruits at different fertility stages. Columns of different colors represent flesh samples from different fertility stages of 'TianYu' or 'TianJu'. The data are shown as the mean ± SD. Different letters indicate statistical significance at p < 0.05 (Duncan's test).

    • Figure 3. 

      Statistics of DEGs in between various comparision groups. (a) Depicts the number of DEGs across the four comparison groups; (b) presents a Venn diagram illustrating the common and unique up-regulated DEGs among different pairwise comparison groups; (c) displays a Venn diagram representing the common and unique down-regulated DEGs across various pairwise comparison groups.

    • Figure 4. 

      qRT-PCR Validation of DEGs in different comparison groups. (a) Represents the quantitative expression results of DEGs in the comparison group of 'TianYu_O vs TianYu_W'; (b) illustrates the quantitative expression results of DEGs in the comparison group of 'TianJu_O vs TianYu_W'; (c) demonstrates the quantitative expression results of DEGs in the comparison group of 'TianJu_O vs TianJu_W'; (d) depicts the quantitative expression results of DEGs in the comparison group of 'TianJu_O vs TianYu_O'. Relative expression (Y-axis) was calculated using the 2−ΔΔCᴛ method, with DEGs shown on the X-axis. Bars represent the mean ± SD of three replicates. * and ** denote significant differences in expression levels at p < 0.05 and p < 0.01, respectively (Student's t-test).

    • Figure 5. 

      GO enrichment analysis of DEGs in the four comparison groups. (a) Top 30 GO terms for the TianJu_O vs TianJu_W comparison; (b) top 30 GO terms for the TianJu_W vs TianYu_W comparison; (c) top 30 GO terms for the TianYu_O vs TianYu_W comparison; (d) top 30 GO terms for the TianJu_O vs TianYu_O comparison. The outermost circle represents the scale for the number of genes, with each division denoting a specific enriched GO classification, differentiated by color. The second circle from the outside displays the number of genes in that GO term within the background gene set, along with the corresponding p-value. The third circle depicts the proportion of up- and down-regulated genes via a bar chart. The innermost circle presents the RichFactor value for each GO term, calculated as the ratio of the number of foreground genes to background genes in that term. Each cell of the background auxiliary line represents an increment of 0.1.

    • Figure 6. 

      KEGG enrichment analysis of DEGs in different comparison groups. (a) Top 20 information under KEGG item about TianJu_O vs TianJu_W comparison group; (b) top 20 information under KEGG item about TianJu_W vs TianYu_W comparison group; (c) top 20 information under KEGG item about TianYu_O vs TianYu_W comparison group; (d) top 20 information under KEGG item about TianJu_O vs TianYu_O comparison group. Bubble size represents the number of DEGs contained under that entry.

    • Figure 7. 

      Differentially expressed TFs across various comparative groups. The four groups of columns, distinguished by color, represent the four comparison groups. The vertical axis denotes the number of differentially expressed TFs, while the horizontal axis enumerates the specific types of TFs.

    • Figure 8. 

      Expression patterns of DEGs involved in carotenoid biosynthesis and metabolism pathways across various comparison groups. Hollow dots signify molecules or products, while green squares denote catalytic enzymes at each stage. Sharp lines indicate synthesis or promotion, and dashed lines represent the presence of other processes. Differentially colored squares represent DEGs, with mRNA color bars illustrating the Log2(FoldChange) values of the DEGs.