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    • Figure 1. 

      Petal colors at different developmental bud stages (PF_L: young bud stage; PF_Z: coloration beginning stage; PF_D: big bud stage) of pink-flowered strawberry cv. 'Sijihong'.

    • Figure 2. 

      Annotation and pathway analysis of differentially expressed genes. (a) The number of differentially expressed genes in the three pairwise comparison groups. (b) Venn diagram showing the intersections of differentially expressed genes in three pairwise comparison groups. (c) The top 20 KEGG significant enrichment pathways of differentially expressed genes in the PF_D vs PF_Z vs PF_L comparison group. (d) The correlation network diagrams of differentially expressed transcription factors in the three comparison groups.

    • Figure 3. 

      Identification of miRNAs and expression analysis of differentially expressed miRNAs during the flower development of pink-flowered strawberry. (a) Location of miRNAs on chromosomes, with known miRNAs in green font, and novel miRNAs in red font. (b) The statistics of the number of identified miRNAs family members. (c) Heatmaps showing expression patterns of the different expression miRNAs during flower development.

    • Figure 4. 

      Screening of key miRNAs-targets. (a) Pathway enrichment analysis of key differentially expressed genes. (b) Correlation network of key miRNAs-targets. (c) The secondary structure prediction of two novel identified miRNAs.

    • Figure 5. 

      Expression pattern analysis of key miRNAs-targets in three developmental stages of 'Sijihong'. (a)–(c) Expression patterns of FamiR156s and their target genes FaSPLs. (d) The expression pattern of FamiR396e and its target gene, transcription initiation factor TFIID subunit 6-like (FaTAF6, FvH4_3g23640). (e) The expression pattern of FamiR11315 and its target gene F-box protein CPR 30-like isoform X3 (FaCPR30, FvH4_3g42390). (f) The expression pattern of novel miR16 and its target gene FaRGA3 (FvH4_6g12200).

    • Figure 6. 

      Identification of PHAS loci and expression analysis of differentially expressed PHAS loci during the flower development of pink-flowered strawberry. (a) Location of PHAS loci on chromosomes, with 21-nt PHAS loci in blue font and 24-nt PHAS loci in orange font. (b) Heatmaps showing expression patterns of the different expression PHAS loci during flower development. (c) PHAS2-108 located on Fvb4 was annotated as transcription factor bHLH77-like, and the results of PHASList to Graph in three developmental stages.

    • Figure 7. 

      FvmiR393a/b triggered phasiRNAs produced from the auxin signaling F-Box 2 (FvFAB2) locus in 'Sijihong'.