Back Article

Mignouna HD, Abang MM, Asiedu R. 2003. Harnessing modern biotechnology for tropical tuber crop improvement: Yam (Dioscorea spp.) molecular breeding. African Journal of Biotechnology 2:478−85

Saski CA, Bhattacharjee R, Scheffler BE. 2015. Genomic resources for water yam (Dioscorea alata L.): analyses of EST-sequences, de novo sequencing and GBS libraries. PLoS One 10:e0134031

Wang X, Chen D, Wang Y, Xie J. 2015. De novo transcriptome assembly and the putative biosynthetic pathway of steroidal sapogenins of Dioscorea composita. PLoS One 10:e0124560

Wu ZG, Jiang W, Mantri N, Bao XQ, Chen SL, et al. 2015. Transciptome analysis reveals flavonoid biosynthesis regulation and simple sequence repeats in yam (Dioscorea alata L.) tubers. BMC Genomics 16:346

Tamiru M, Natsume S, Takagi H, White B, Yaegashi H, et al. 2017. Genome sequencing of the staple food crop white Guinea yam enables the development of a molecular marker for sex determination. BMC Biology 15:86

Zhou W, Li B, Li L, Ma W, Liu Y, et al. 2018. Genome survey sequencing of Dioscorea zingiberensis. Genome 61:567−74

Bredeson JV, Lyons JB, Oniyinde IO, Okereke NR, Kolade O, et al. 2022. Chromosome evolution and the genetic basis of agronomically important traits in greater yam. Nature Communications 13:2001

Adeniyi OJ, Adetimirin VO, Ingelbrecht I, Asiedu R. 2008. Shoot and plantlet regeneration from meristems of Dioscorea rotundata Poir and Dioscorea alata L. African Journal of Biotechnology 7:1003−8

Anike FN, Konan K, Olivier K, Dodo H. 2012. Efficient shoot organogenesis in petioles of yam (Dioscorea spp). Plant Cell Tissue and Organ Culture 111:303−13

Nyaboga E, Tripathi JN, Manoharan R, Tripathi L. 2014. Agrobacterium-mediated genetic transformation of yam (Dioscorea rotundata): an important tool for functional study of genes and crop improvement. Frontiers in Plant Science 5:463

Siefritz F, Biela A, Eckert M, Otto B, Uehlein N, et al. 2001. The tobacco plasma membrane aquaporin NtAQP1. Journal of Experimental Botany 52:1953−57

Wei W, Wu K, Qin Y, Xie Z, Zhu X. 2001. Intergeneric protoplast fusion between Kluyveromyces and Saccharomyces cerevisiae – to produce sorbitol from Jerusalem artichokes. Biotechnology Letters 23:799−803

Yoo SD, Cho YH, Sheen J. 2007. Arabidopsis mesophyll protoplasts: a versatile cell system for transient gene expression analysis. Nature Protocols 2:1565−72

Mazarei M, Al-Ahmad H, Rudis MR, Jr Stewart CN. 2008. Protoplast isolation and transient gene expression in switchgrass, Panicum virgatum L. Biotechnol Journal 3:354−59

Chen S, Tao L, Zeng L, Vegasanchez ME, Umemura K, et al. 2010. A highly efficient transient protoplast system for analyzing defence gene expression and protein-protein interactions in rice. Molecular Plant Pathology 7:417−27

Johnson EE, Jetter R, Wasteneys GO. 2014. Rapid induction of the triterpenoid pathway in Arabidopsis thaliana mesophyll protoplasts. Biotechnology Letters 36:855−58

Martinho C, Confraria A, Elias CA, Crozet P, Rubiosomoza I, et al. 2014. Dissection of miRNA pathways using Arabidopsis mesophyll protoplasts. Molecular Plant 8:261−75

Kokkirala VR, Peng Y, Abbagani S, Zhu Z, Umate P. 2010. Subcellular localization of proteins of Oryza sativa L. in the model tobacco and tomato plants. Plant Signaling & Behavior 5:1336−41

Zhou Y, Zhou H, Lin-Wang K, Vimolmangkang S, Espley RV, et al. 2014. Transcriptome analysis and transient transformation suggest an ancient duplicated MYB transcription factor as a candidate gene for leaf red coloration in peach. BMC Plant Biology 14:1−13

Liu L, Huang Y, Huang X, Yang J, Wu W, et al. 2017. Characterization of the dioscorin gene family in Dioscorea alata reveals a role in tuber development and environmental response. International Journal of Molecular Sciences 18:1579

Faraco M, Sansebastiano GPD, Spelt K, Koes R, Quattrocchio F. 2011. One protoplast is not the other. Plant Physiology 156:474−78

Tor M, Twyford CT, Funes I, Boccon-Gibod J, Ainsworth CC, et al. 1998. Isolation and culture of protoplasts from immature leaves and embryogenic cell suspensions of Dioscorea yams: tools for transient gene expression studies. Plant Cell, Tissue and Organ Culture 53:113−26

Zhang J, Zhang B, Huang D, Xiao Y, Wu W, et al. 2019. Identification and Characterization of ERF Gene Family in Great Yam (Dioscorea alata L.). Molecular Plant Breeding 17:2086−93

Xiao Y, Yang Y, Cao H, Fan H, Ma Z, et al. 2012. Efficient isolation of high quality RNA from tropical palms for RNA-seq analysis. Plant Omics 5:584−89

Wu JZ, Liu Q, Geng XS, Li KM, Luo LJ, et al. 2017. Highly efficient mesophyll protoplast isolation and PEG-mediated transient gene expression for rapid and large-scale gene characterization in cassava (Manihot esculenta Crantz). BMC Biotechnology 17:29

Jeon JM, Ahn NY, Son BH, Kim CY, Han CD, et al. 2007. Efficient transient expression and transformation of PEG-mediated gene uptake into mesophyll protoplasts of pepper (Capsicum annuum L.). Plant Cell, Tissue and Organ Culture 88:225−32

Bai Y, Han N, Wu J, Yang Y, Wang J, et al. 2014. A transient gene expression system using barley protoplasts to evaluate microRNAs for post-transcriptional regulation of their target genes. Plant Cell Tissue and Organ Culture 119:211−19

Nanjareddy K, Arthikala MK, Blanco L, Arellano ES, Lara M. 2016. Protoplast isolation, transient transformation of leaf mesophyll protoplasts and improved Agrobacterium-mediated leaf disc infiltration of Phaseolus vulgaris: tools for rapid gene expression analysis. BMC Biotechnology 16:53

Yu G, Cheng Q, Xie Z, Xu B, Huang B, et al. 2017. An efficient protocol for perennial ryegrass mesophyll protoplast isolation and transformation, and its application on interaction study between LpNOL and LpNYC1. Plant Methods 13:46