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This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Comparative chloroplast genome analysis of Camellia oleifera and C. meiocarpa: phylogenetic relationships, sequence variation and polymorphism markers

  • 1.

    National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, 572024, China

  • 2.

    Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572025, China

  • 3.

    Institute of Tropical Horticulture Research, Hainan Academy of Agricultural Sciences, Haikou 571100, China

  • 4.

    Key Laboratory of Tropic Special Economic Plant Innovation and Utilization, Haikou 571100, China

  • 5.

    National Germplasm Resource Chengmai Observation and Experiment Station, Chengmai 571100, China

  • 6.

    Precision Agriculture Laboratory, School of Life Sciences, Technical University of Munich, Freising 85354, Germany

  • 7.

    Guangxi Key Laboratory of Special Non-Wood Forest Cultivation and Utilization, Improved Variety and Cultivation Engineering Research Center of Oil-Tea Camellia in Guangxi, Guangxi Forestry Research Institute, Nanning 530002, China

  • # Authors contributed equally: Heng Liang, Huasha Qi

More Information

  • Compared to C. oleifera (HZP), there were differences ranging between 460 bp (CKX) and 490 bp (XG) in C. meiocarpa.

    C. meiocarpa was considered as separated species.

    The development of 17 primers could be used for the resource assessment of Camellia.

  • Tea-oil Camellia, a prominently woody oil crop, serves as a crucial source of edible oil, protein feed, and industrial raw materials. Notably, C. Oleifera and C. meiocarpa yield higher oil production and larger cultivation areas than other Tea-oil Camellia species. However, the taxonomy and phylogenetic relationship between these species remain elusive, complicating their commercial application. Here, we sequenced and analyzed the complete chloroplast genomes of these two species, compared them with related Camellia species, and developed chloroplast DNA markers to distinguish between them. The chloroplast genome of C. Oleifera was 157,009 bp (HZP) and C. meiocarpa was 156,549 bp (CKX) and 156,512 bp (XG) in length. Comparative analysis indicated that distinct differences in the chloroplast genome between HZP and CKX (or XG) than between CKX and XG. The repetitive sequences and interspecific variations among them showed that the differences in number and distribution of CKX and XG were smaller than those in HZP. Phylogenetic analysis showed that C. meiocarpa was not closely related to C. oleifera. A total of 56 pairs of primers were developed to test the polymorphism among them. After PCR and sequencing verification, variations were detected in the target sequences of 17 primers. The data derived from the chloroplast genomes and the newly developed markers are invaluable for understanding the phylogenetic relationships, and assessing the genetic diversity of tea-oil Camellia germplasm resources.
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  • Cite this article

    Liang H, Qi H, Wang Y, Sun X, Wang C, et al. 2024. Comparative chloroplast genome analysis of Camellia oleifera and C. meiocarpa: phylogenetic relationships, sequence variation and polymorphism markers. Tropical Plants doi: 10.48130/tp-0024-0022
    Liang H, Qi H, Wang Y, Sun X, Wang C, et al. 2024. Comparative chloroplast genome analysis of Camellia oleifera and C. meiocarpa: phylogenetic relationships, sequence variation and polymorphism markers. Tropical Plants doi: 10.48130/tp-0024-0022
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Important Notice

This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Comparative chloroplast genome analysis of Camellia oleifera and C. meiocarpa: phylogenetic relationships, sequence variation and polymorphism markers

  • 1.

    National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya, 572024, China

  • 2.

    Sanya Institute, Hainan Academy of Agricultural Sciences, Sanya 572025, China

  • 3.

    Institute of Tropical Horticulture Research, Hainan Academy of Agricultural Sciences, Haikou 571100, China

  • 4.

    Key Laboratory of Tropic Special Economic Plant Innovation and Utilization, Haikou 571100, China

  • 5.

    National Germplasm Resource Chengmai Observation and Experiment Station, Chengmai 571100, China

  • 6.

    Precision Agriculture Laboratory, School of Life Sciences, Technical University of Munich, Freising 85354, Germany

  • 7.

    Guangxi Key Laboratory of Special Non-Wood Forest Cultivation and Utilization, Improved Variety and Cultivation Engineering Research Center of Oil-Tea Camellia in Guangxi, Guangxi Forestry Research Institute, Nanning 530002, China

  • Received Date: 21 March 2024
  • Accepted Date: 26 April 2024
    • First-online Date: 10 May 2024
Tropical Plants  Article in press  (2024)  |  Cite this article

Abstract: Tea-oil Camellia, a prominently woody oil crop, serves as a crucial source of edible oil, protein feed, and industrial raw materials. Notably, C. Oleifera and C. meiocarpa yield higher oil production and larger cultivation areas than other Tea-oil Camellia species. However, the taxonomy and phylogenetic relationship between these species remain elusive, complicating their commercial application. Here, we sequenced and analyzed the complete chloroplast genomes of these two species, compared them with related Camellia species, and developed chloroplast DNA markers to distinguish between them. The chloroplast genome of C. Oleifera was 157,009 bp (HZP) and C. meiocarpa was 156,549 bp (CKX) and 156,512 bp (XG) in length. Comparative analysis indicated that distinct differences in the chloroplast genome between HZP and CKX (or XG) than between CKX and XG. The repetitive sequences and interspecific variations among them showed that the differences in number and distribution of CKX and XG were smaller than those in HZP. Phylogenetic analysis showed that C. meiocarpa was not closely related to C. oleifera. A total of 56 pairs of primers were developed to test the polymorphism among them. After PCR and sequencing verification, variations were detected in the target sequences of 17 primers. The data derived from the chloroplast genomes and the newly developed markers are invaluable for understanding the phylogenetic relationships, and assessing the genetic diversity of tea-oil Camellia germplasm resources.

    Acknowledgments

    • This study was supported by the Project of Sanya Yazhou Bay Science and Technology City [grant number SCKJ-JYRC-202258], Southen Breeding Project of Sanya National Southen Breeding Research Academy of Chinese Academy of Agricultural Sciences (No.YYLH10), the National Natural Science Foundation of China (31860082), Hainan Province Science and Technology Special Fund (FW20230002), Scientific and technological innovation team of Hainan Academy of Agricultural Sciences (HAAS2023TDYD05)， introduce talents to initiate scientific research projects of Hainan Academy of Agricultural Sciences (HAAS2023RCQD13).

    Conflict of interest

    • The authors declare that they have no conflict of interest.

    Supplementary Information

    • accompanies this paper at (XXXXXX)

    Dates

    • Received 21 March 2024; Accepted 26 April 2024; Published online xxxxxx

    Highlights

    • Compared to C. oleifera (HZP), there were differences ranging between 460 bp (CKX) and 490 bp (XG) in C. meiocarpa.

      C. meiocarpa was considered as separated species.

      The development of 17 primers could be used for the resource assessment of Camellia.

    • # Authors contributed equally: Heng Liang, Huasha Qi

    Supplementary Information
    Rights and permissions
    • Copyright: © 2024 by the author(s). Published by Maximum Academic Press on behalf of Hainan University. This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
Figure (6)  Table (4)
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    Cite this article
    Liang H, Qi H, Wang Y, Sun X, Wang C, et al. 2024. Comparative chloroplast genome analysis of Camellia oleifera and C. meiocarpa: phylogenetic relationships, sequence variation and polymorphism markers. Tropical Plants doi: 10.48130/tp-0024-0022
    Liang H, Qi H, Wang Y, Sun X, Wang C, et al. 2024. Comparative chloroplast genome analysis of Camellia oleifera and C. meiocarpa: phylogenetic relationships, sequence variation and polymorphism markers. Tropical Plants doi: 10.48130/tp-0024-0022
    shu

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