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This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Abscisic acid participates in melatonin-induced chilling tolerance of cucumber via regulating photosynthesis and antioxidant system

  • 1.

    Key Laboratory of Crop Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, Shandong, 271018 China

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  • Melatonin (MT) and abscisic acid (ABA) are crucial in regulating abiotic stress tolerance in cucumber. However, their roles as signaling molecules in regulating chilling tolerance remain ambiguous. The results showed that neither applying ABA nor removing endogenous ABA with sodium tungstate (Na2WO4, an inhibitor of ABA synthesis) has no effect on endogenous MT levels, however, MT induced endogenous ABA content via activating the activities and mRNA levels of 9-cis-epoxycarotenoid dioxygenase (NCED) under normal conditions and inhibiting endogenous MT with p-chlorophenylalanine (p-CPA) blocked this effect. Further studies indicated that MT and ABA had a noteworthy effect on enhancing the chilling tolerance of cucumber seedlings in terms of lower chilling injury, EL and MDA contents. Moreover, cucumber seedlings pretreated with MT and ABA showed lower ROS contents and higher antioxidant capacity than those in H2O treatment under chilling stress. Meanwhile, MT and ABA alleviated the decline of chlorophyll content, photosynthesis, the gene expression and protein level of Rubisco and RCA caused by chilling stress as well as maintained photosynthetic electron transport via increasing heat dissipation, the levels of proteins PsbS and VDE as well as decreasing the chilling damage to donor and recipient side of electron transport chain under chilling stress. However, it was discovered that Na2WO4 and fluridone (Flu) blocked the chilling tolerance induced by MT, whereas p-CPA had little impact on ABA promotion on chilling tolerance of cucumber. These findings imply that ABA may act as a downstream signal of MT in the regulation of chilling tolerance in cucumber.

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    Feng Y, Liu C, Gong B, Ai X, Bi H. 2024. Abscisic acid participates in melatonin-induced chilling tolerance of cucumber via regulating photosynthesis and antioxidant system. Vegetable Research doi: 10.48130/vegres-0024-0024
    Feng Y, Liu C, Gong B, Ai X, Bi H. 2024. Abscisic acid participates in melatonin-induced chilling tolerance of cucumber via regulating photosynthesis and antioxidant system. Vegetable Research doi: 10.48130/vegres-0024-0024
    shu

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Important Notice

This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Abscisic acid participates in melatonin-induced chilling tolerance of cucumber via regulating photosynthesis and antioxidant system

  • 1.

    Key Laboratory of Crop Biology and Genetic Improvement of Horticultural Crops in Huanghuai Region; College of Horticulture Science and Engineering, Shandong Agricultural University, Tai’an, Shandong, 271018 China

  • Received Date: 10 May 2024
  • Accepted Date: 11 June 2024
    • First-online Date: 23 June 2024
Vegetable Research  Article in press  (2024)  |  Cite this article

Abstract: Melatonin (MT) and abscisic acid (ABA) are crucial in regulating abiotic stress tolerance in cucumber. However, their roles as signaling molecules in regulating chilling tolerance remain ambiguous. The results showed that neither applying ABA nor removing endogenous ABA with sodium tungstate (Na2WO4, an inhibitor of ABA synthesis) has no effect on endogenous MT levels, however, MT induced endogenous ABA content via activating the activities and mRNA levels of 9-cis-epoxycarotenoid dioxygenase (NCED) under normal conditions and inhibiting endogenous MT with p-chlorophenylalanine (p-CPA) blocked this effect. Further studies indicated that MT and ABA had a noteworthy effect on enhancing the chilling tolerance of cucumber seedlings in terms of lower chilling injury, EL and MDA contents. Moreover, cucumber seedlings pretreated with MT and ABA showed lower ROS contents and higher antioxidant capacity than those in H2O treatment under chilling stress. Meanwhile, MT and ABA alleviated the decline of chlorophyll content, photosynthesis, the gene expression and protein level of Rubisco and RCA caused by chilling stress as well as maintained photosynthetic electron transport via increasing heat dissipation, the levels of proteins PsbS and VDE as well as decreasing the chilling damage to donor and recipient side of electron transport chain under chilling stress. However, it was discovered that Na2WO4 and fluridone (Flu) blocked the chilling tolerance induced by MT, whereas p-CPA had little impact on ABA promotion on chilling tolerance of cucumber. These findings imply that ABA may act as a downstream signal of MT in the regulation of chilling tolerance in cucumber.

    Acknowledgments

    • This work was supported by the National Key Research and Development Program of China (2018YFD1000800); the National Science Foundation of Shandong province (ZR2023MC183); the Special Fund of Modern Agriculture Industrial Technology System of Shandong Province in China (SDAIT-05-10).

    Conflict of interest

    • The authors declare that they have no conflict of interest.

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    • Copyright: © 2024 by the author(s). Published by Maximum Academic Press, Fayetteville, GA. This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
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    Cite this article
    Feng Y, Liu C, Gong B, Ai X, Bi H. 2024. Abscisic acid participates in melatonin-induced chilling tolerance of cucumber via regulating photosynthesis and antioxidant system. Vegetable Research doi: 10.48130/vegres-0024-0024
    Feng Y, Liu C, Gong B, Ai X, Bi H. 2024. Abscisic acid participates in melatonin-induced chilling tolerance of cucumber via regulating photosynthesis and antioxidant system. Vegetable Research doi: 10.48130/vegres-0024-0024
    shu

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