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This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

METHOD   Open Access    

Efficient detection of melon-powdery mildew interactions by a medium-free inoculation

  • 1.

    College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China

  • 2.

    Agricultural Science and Engineering School, Liaocheng University, Liaocheng, Shandong 252000, China

  • # Authors contributed equally: Jianquan Wang, Shuoshuo Wang

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  • To investigate the interplay between host plants and fungal pathogens, two inoculation strategies, liquid-mediated (LM) and medium-free (MF) ones, are commonly used. For LM strategy, host plants are infected by spraying of target pathogens that are suspended in proper solutions, while the collected pathogens are directly blown onto host plants for MF inoculation. In contrast to widespread application of LM strategy, MF inoculation has never been adopted in previous studies about melon-powdery mildew interactions. In this study, an MF strategy suitable for Cucumis melo L. was developed, and its effectiveness was evaluated with the seedlings of inbred line 'YJM'. qPCR results showed that, in comparison to the LM group, the germination and growth of Podosphaera xanthii (P. xanthii) pathogens were apparently promoted, and therefore, the disease symptoms were able to be detected more efficiently on the leaves of melon seedlings that were treated via MF inoculation. This stimulated pathogen development on MF-treated leaves was further supported by microscopic observations relative to LM-treated ones, revealing the great potential of MF inoculation in high-efficiency detection of the interplay between melon and P. xanthii. Altogether, our results shed new methodological insights into melon-powdery mildew interactions and could contribute to pathogenicity assays in this field.

  • Cite this article

    Wang J, Wang S, Guo Y, Hu Z, Yin M, et al. 2024. Efficient detection of melon-powdery mildew interactions by a medium-free inoculation. Vegetable Research doi: 10.48130/vegres-0024-0022
    Wang J, Wang S, Guo Y, Hu Z, Yin M, et al. 2024. Efficient detection of melon-powdery mildew interactions by a medium-free inoculation. Vegetable Research doi: 10.48130/vegres-0024-0022
    shu

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Important Notice

This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

METHOD   Open Access    

Efficient detection of melon-powdery mildew interactions by a medium-free inoculation

  • 1.

    College of Horticultural Science and Engineering, Shandong Agricultural University, Tai'an, Shandong 271018, China

  • 2.

    Agricultural Science and Engineering School, Liaocheng University, Liaocheng, Shandong 252000, China

  • Received Date: 23 February 2024
  • Accepted Date: 11 June 2024
    • First-online Date: 14 June 2024
Vegetable Research  Article in press  (2024)  |  Cite this article

Abstract: To investigate the interplay between host plants and fungal pathogens, two inoculation strategies, liquid-mediated (LM) and medium-free (MF) ones, are commonly used. For LM strategy, host plants are infected by spraying of target pathogens that are suspended in proper solutions, while the collected pathogens are directly blown onto host plants for MF inoculation. In contrast to widespread application of LM strategy, MF inoculation has never been adopted in previous studies about melon-powdery mildew interactions. In this study, an MF strategy suitable for Cucumis melo L. was developed, and its effectiveness was evaluated with the seedlings of inbred line 'YJM'. qPCR results showed that, in comparison to the LM group, the germination and growth of Podosphaera xanthii (P. xanthii) pathogens were apparently promoted, and therefore, the disease symptoms were able to be detected more efficiently on the leaves of melon seedlings that were treated via MF inoculation. This stimulated pathogen development on MF-treated leaves was further supported by microscopic observations relative to LM-treated ones, revealing the great potential of MF inoculation in high-efficiency detection of the interplay between melon and P. xanthii. Altogether, our results shed new methodological insights into melon-powdery mildew interactions and could contribute to pathogenicity assays in this field.

    Acknowledgments

    • This work was supported by National Natural Science Foundation of China (32272795), the Taishan Scholar Youth Expert Program of Shandong Province (tsqnz20230605), Natural Science Foundation of Shandong Province (ZR2022MC029), and the Agricultural Variety Improvement Project of Shandong Province (2022LZGCQY006).

    Conflict of interest

    • The authors declare that they have no conflict of interest.

    • # Authors contributed equally: Jianquan Wang, Shuoshuo Wang

    Rights and permissions
    • Copyright: © 2024 by the author(s). Published by Maximum Academic Press, Fayetteville, GA. This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
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    Cite this article
    Wang J, Wang S, Guo Y, Hu Z, Yin M, et al. 2024. Efficient detection of melon-powdery mildew interactions by a medium-free inoculation. Vegetable Research doi: 10.48130/vegres-0024-0022
    Wang J, Wang S, Guo Y, Hu Z, Yin M, et al. 2024. Efficient detection of melon-powdery mildew interactions by a medium-free inoculation. Vegetable Research doi: 10.48130/vegres-0024-0022
    shu

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