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This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Multi-explant and multiplex applications of plant growth regulators: a critical analysis of direct organogenesis in Gloriosa superba (L.)

  • 1.

    Centre for Biodiversity Exploration and Conservation (CBEC), 15, Kundan Residency, 4th Mile Mandla Road, Tilhari, Jabalpur, 482021, M.P, India

  • 2.

    School of Sciences, Sanjeev Agrawal Global Educational (SAGE) University, Bhopal, M.P, 462022, India

  • 3.

    Bhoomi Institute of Research in Advance Biotechnology (BIRAB), Plot No. Z-20, SF-5, A-2, Badri Mahal, M.P. Nagar, Zone – I, Bhopal (M.P.) – 462011, India

  • 4.

    Institute of Biological Science, Sanjeev Agrawal Global Educational (SAGE) University, Indore, M.P, 452020, India

  • 5.

    Environmental Horticulture Department, University of Florida, Institute of Food and Agricultural Sciences, 2550 Hull Rd., Gainesville, FL 32611, USA

More Information

  • This study presents the first report on a multi-plant approach for in vitro plant regeneration through direct organogenesis in Gloriosa superba L.

    The study found that a concentration of 1.5 mg L−1 of BAP was the most effective for inducing shoots across all five types of explants.

    Optimal TDZ concentration was observed to expedite the response to shoot induction.

    In combined treatments, ADS was found to effectively promote shoot proliferation and enhance the effects of other plant growth regulators.

    The highest rate of rooting response in microshoots was achieved when the rooting media was supplemented with 1.0 mg L−1 of IBA.

    This multi-explant in vitro propagation approach is recommended for wider conservation efforts using plant tissue culture.

  • Gloriosa superba L., commonly known as glory lily, is a monocotyledonous plant with both ornamental and medicinal value. In this study, the objective was to develop a reliable and reproducible technique for inducing organogenic bud formation from various explants of the glory lily. We investigated the effects of different types and combinations of plant growth regulators (PGRs) on in vitro plant regeneration using Murashige and Skoog medium (MS) across different explant types. This study established protocols for shoot induction and plant regeneration using apical shoot, meristem, shoot tip, nodal segment, and non-dormant corm explants of Gloriosa superba L. For nodal explants, the highest shoot induction rate of 88.89% was achieved with 1.5 mg L−1 6-Benzylaminopurine (BAP) and 0.2 mg L−1 Thidiazuron (TDZ), with shoots forming within 8 days. Non-dormant corm explants demonstrated the highest shoot induction rate of 91.67% when treated with 1.5 mg L−1 BAP and 10 mg L−1 Adenine Sulfate (ADS), with shoots forming within 7 days. For shoot tip explants, a maximum shoot induction rate of 86.11% was observed with a combination of 1.5 mg L−1 BAP, 0.2 mg L−1 TDZ, and 8 mg L−1 ADS, with shoot formation occurring within 6 days. Apical shoot explants showed an 85.41% shoot induction rate when supplemented with 1.5 mg L−1 BAP and 0.2 mg L−1 1-Naphthaleneacetic acid (NAA), with shoots forming within 8.25 days. Finally, meristem explants achieved a maximum shoot induction rate of 89.58% with 1.5 mg L−1 BAP and 0.2 mg L−1 NAA, with shoots forming within 7 days. All rooting treatments successfully induced root formation, with the most effective results observed on half-strength MS medium supplemented with 1.0 mg L−1 IBA. This treatment achieved the highest rooting response rate of 81.25% and the longest average root length of 4.64 cm. The in vitro-grown plantlets were effectively acclimatised and transplanted into a garden soil mixture of sand and vermiculite (2:1:1, v/v) under direct sunlight, achieving a survival rate of 60% after ten weeks. This study underscores the significance of a multi-explant in vitro regeneration system for the conservation of Gloriosa superba L., emphasizing the strategic application of plant growth regulators and the process of direct organogenesis. The findings offer a comprehensive framework for the sustainable management and preservation of this species.
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  • Cite this article

    Mosoh DA, Khandel AK, Verma SK, Vendram WA. 2024. Multi-explant and multiplex applications of plant growth regulators: a critical analysis of direct organogenesis in Gloriosa superba (L.). Tropical Plants doi: 10.48130/tp-0024-0038
    Mosoh DA, Khandel AK, Verma SK, Vendram WA. 2024. Multi-explant and multiplex applications of plant growth regulators: a critical analysis of direct organogenesis in Gloriosa superba (L.). Tropical Plants doi: 10.48130/tp-0024-0038
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Important Notice

This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Multi-explant and multiplex applications of plant growth regulators: a critical analysis of direct organogenesis in Gloriosa superba (L.)

  • 1.

    Centre for Biodiversity Exploration and Conservation (CBEC), 15, Kundan Residency, 4th Mile Mandla Road, Tilhari, Jabalpur, 482021, M.P, India

  • 2.

    School of Sciences, Sanjeev Agrawal Global Educational (SAGE) University, Bhopal, M.P, 462022, India

  • 3.

    Bhoomi Institute of Research in Advance Biotechnology (BIRAB), Plot No. Z-20, SF-5, A-2, Badri Mahal, M.P. Nagar, Zone – I, Bhopal (M.P.) – 462011, India

  • 4.

    Institute of Biological Science, Sanjeev Agrawal Global Educational (SAGE) University, Indore, M.P, 452020, India

  • 5.

    Environmental Horticulture Department, University of Florida, Institute of Food and Agricultural Sciences, 2550 Hull Rd., Gainesville, FL 32611, USA

  • Received: 29 May 2024
  • Revised: 12 August 2024
  • Accepted: 12 September 2024
    • First-online: 21 September 2024
Tropical Plants  Article in press  ()  |  Cite this article

Abstract: Gloriosa superba L., commonly known as glory lily, is a monocotyledonous plant with both ornamental and medicinal value. In this study, the objective was to develop a reliable and reproducible technique for inducing organogenic bud formation from various explants of the glory lily. We investigated the effects of different types and combinations of plant growth regulators (PGRs) on in vitro plant regeneration using Murashige and Skoog medium (MS) across different explant types. This study established protocols for shoot induction and plant regeneration using apical shoot, meristem, shoot tip, nodal segment, and non-dormant corm explants of Gloriosa superba L. For nodal explants, the highest shoot induction rate of 88.89% was achieved with 1.5 mg L−1 6-Benzylaminopurine (BAP) and 0.2 mg L−1 Thidiazuron (TDZ), with shoots forming within 8 days. Non-dormant corm explants demonstrated the highest shoot induction rate of 91.67% when treated with 1.5 mg L−1 BAP and 10 mg L−1 Adenine Sulfate (ADS), with shoots forming within 7 days. For shoot tip explants, a maximum shoot induction rate of 86.11% was observed with a combination of 1.5 mg L−1 BAP, 0.2 mg L−1 TDZ, and 8 mg L−1 ADS, with shoot formation occurring within 6 days. Apical shoot explants showed an 85.41% shoot induction rate when supplemented with 1.5 mg L−1 BAP and 0.2 mg L−1 1-Naphthaleneacetic acid (NAA), with shoots forming within 8.25 days. Finally, meristem explants achieved a maximum shoot induction rate of 89.58% with 1.5 mg L−1 BAP and 0.2 mg L−1 NAA, with shoots forming within 7 days. All rooting treatments successfully induced root formation, with the most effective results observed on half-strength MS medium supplemented with 1.0 mg L−1 IBA. This treatment achieved the highest rooting response rate of 81.25% and the longest average root length of 4.64 cm. The in vitro-grown plantlets were effectively acclimatised and transplanted into a garden soil mixture of sand and vermiculite (2:1:1, v/v) under direct sunlight, achieving a survival rate of 60% after ten weeks. This study underscores the significance of a multi-explant in vitro regeneration system for the conservation of Gloriosa superba L., emphasizing the strategic application of plant growth regulators and the process of direct organogenesis. The findings offer a comprehensive framework for the sustainable management and preservation of this species.

    Acknowledgments

    • The USDA National Institute of Food and Agriculture has provided support for this study, specifically under the Hatch project 7001563. The corresponding author thanks Chief MOSOH Paul Tandong and Chieftess Ateyim Espe MOSOH Ostensia Nkeng of PINYIN (Santa, North-West Region, Cameroon) for their tremendous support. Dr. Rohit Sharma, the founder of the Centre for Biodiversity Exploration and Conservation (CBEC), provided invaluable assistance at the start of this project. His contributions have significantly influenced the direction of our research. Additionally, we express our sincere appreciation to the reviewer(s) for their diligent review of our work, and for their insightful comments and suggestions, which have significantly enriched the quality of our manuscript.

    Conflict of interest

    • The authors declare that they have no conflict of interest.

    Supplementary Information

    • accompanies this paper at (XXXXXX)

    Dates

    • Received 29 May 2024; Accepted 12 September 2024; Published online xxxxxx

    Highlights

    • This study presents the first report on a multi-plant approach for in vitro plant regeneration through direct organogenesis in Gloriosa superba L.

      The study found that a concentration of 1.5 mg L−1 of BAP was the most effective for inducing shoots across all five types of explants.

      Optimal TDZ concentration was observed to expedite the response to shoot induction.

      In combined treatments, ADS was found to effectively promote shoot proliferation and enhance the effects of other plant growth regulators.

      The highest rate of rooting response in microshoots was achieved when the rooting media was supplemented with 1.0 mg L−1 of IBA.

      This multi-explant in vitro propagation approach is recommended for wider conservation efforts using plant tissue culture.

    Supplementary information
    Rights and permissions
    • Copyright: © 2024 by the author(s). Published by Maximum Academic Press on behalf of Hainan University. This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
Figure (15)  Table (5)
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    Cite this article
    Mosoh DA, Khandel AK, Verma SK, Vendram WA. 2024. Multi-explant and multiplex applications of plant growth regulators: a critical analysis of direct organogenesis in Gloriosa superba (L.). Tropical Plants doi: 10.48130/tp-0024-0038
    Mosoh DA, Khandel AK, Verma SK, Vendram WA. 2024. Multi-explant and multiplex applications of plant growth regulators: a critical analysis of direct organogenesis in Gloriosa superba (L.). Tropical Plants doi: 10.48130/tp-0024-0038
    shu

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