2022 Volume 2
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Growth and physiological effects of chitosan on heat tolerance in creeping bentgrass (Agrostis stolonifera)

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  • High temperature is one of the major abiotic stresses limiting growth and development of cool-season grass species, but chitosan could effectively enhance heat tolerance and improve plant growth. The objective of this study was to determine the optimal concentration of chitosan that could alleviate heat stress in creeping bentgrass (Agrostis stolonifera) and investigate the effects of exogenous chitosan on photosynthesis and cell membrane stability under heat stress. Under heat stress (38/28 °C, day/night), different chitosan concentrations of 0, 50, 100 and 500 mg·L−1 were applied on the leaves of creeping bentgrass (cv. 'Penn-A4'). Foliar application of chitosan exhibited the positive effects on plant growth and the optimal concentration was 100 mg·L−1 which significantly improved turf quality, root length, root-shoot ratio as well as shoot and root biomass. Chitosan-treated plants subjected to high temperature stress had a lower decline in photosynthetic rate and photochemical efficiency as well as less increase in electrolyte leakage and malondialdehyde content. The results demonstrate that chitosan-improved heat tolerance as reflected by the superior growth performance of both shoot and root, photosynthesis and cell membrane stability in creeping bentgrass under heat stress.
  • Columnar cacti are plants of the Cactaceae family distributed across arid and semi-arid regions of America, with ecological, economic, and cultural value[1]. One trait that makes it possible for the columnar cactus to survive in the desert ecosystem is its thick epidermis covered by a hydrophobic cuticle, which limits water loss in dry conditions[1]. The cuticle is the external layer that covers the non-woody aerial organs of land plants. The careful control of cuticle biosynthesis could produce drought stress tolerance in relevant crop plants[2]. In fleshy fruits, the cuticle maintains adequate water content during fruit development on the plant and reduces water loss in fruit during postharvest[3]. Efforts to elucidate the molecular pathway of cuticle biosynthesis have been carried out for fleshy fruits such as tomato (Solanum lycopersicum)[4], apple (Malus domestica)[5], sweet cherry (Prunus avium)[6], mango (Mangifera indica)[7], and pear (Pyrus 'Yuluxiang')[8].

    The plant cuticle is formed by the two main layers cutin and cuticular waxes[3]. Cutin is composed mainly of oxygenated long-chain (LC) fatty acids (FA), which are synthesized by cytochrome p450 (CYP) enzymes. CYP family 86 subfamily A (CYP86A) enzymes carry out the terminal (ω) oxidation of LC-FA[9]. Then, CYP77A carries out the mid-chain oxidation to synthesize the main cutin monomers. In Arabidopsis, AtCYP77A4 and AtCYP77A6 carry out the synthesis of mid-chain epoxy and mid-chain dihydroxy LC-FA, respectively[10,11]. AtCYP77A6 is required for the cutin biosynthesis and the correct formation of floral surfaces[10]. The expression of CYP77A19 (KF410855) and CYP77A20 (KF410856) from potato (Solanum tuberosum) restored the petal cuticular impermeability in Arabidopsis null mutant cyp77a6-1, tentatively by the synthesis of cutin monomers[12]. In eggplant (Solanum torvum), the over-expression of StoCYP77A2 leads to resistance to Verticillium dahlia infection in tobacco plants[13]. Although the function of CYP77A2 in cutin biosynthesis has not yet been tested, gene expression analysis suggests that CaCYP77A2 (A0A1U8GYB0) could play a role in cutin biosynthesis during pepper fruit development[14].

    It has been hypothesized that the export of cuticle precursors is carried out by ATP binding cassette subfamily G (ABCG) transporters. ABCG11/WBC11, ABCG12, and ABCG13 are required for the load of cuticle lipids in Arabidopsis[1517], but ABCG13 function appears to be specific to the flower epidermis[18]. The overexpression of TsABCG11 (JQ389853) from Thellungiella salsugineum increases cuticle amounts and promotes tolerance to different abiotic stresses in Arabidopsis[19].

    Once exported, the cutin monomers are polymerized on the surface of epidermal cells. CD1 code for a Gly-Asp-Ser-Leu motif lipase/esterase (GDSL) from tomato required for the cutin formation through 2-mono(10,16-dihydroxyhexadecanoyl)glycerol esterification[20]. GDSL1 from tomato carries out the ester bond cross-links of cutin monomers located at the cuticle layers and is required for cuticle deposition in tomato fruits[21]. It has been shown that the transcription factor MIXTA-like reduces water loss in tomato fruits through the positive regulation of the expression of CYP77A2, ABCG11, and GDSL1[22]. Despite the relevant role of cuticles in maintaining cactus homeostasis in desert environments[1], the molecular mechanism of cuticle biosynthesis has yet to be described for cactus fruits.

    Stenocereus thurberi is a columnar cactus endemic from the Sonoran desert (Mexico), which produces an ovoid-globose fleshy fruit named sweet pitaya[23]. In its mature state, the pulp of sweet pitaya contains around 86% water with a high content of antioxidants and natural pigments such as betalains and phenolic compounds, which have nutraceutical and industrial relevance[23]. Due to the arid environment in which pitaya fruit grows, studying its molecular mechanism of cuticle biosynthesis can generate new insights into understanding species' adaptation mechanisms to arid environments. Nevertheless, sequences of transcripts from S. thurberi in public databases are scarce.

    RNA-sequencing technology (RNA-seq) allows the massive generation of almost all the transcripts from non-model plants, even if no complete assembled genome is available[24]. Recent advances in bioinformatic tools has improved our capacity to identify long non-coding RNA (lncRNA), which have been showed to play regulatory roles in relevant biological processes, such as the regulation of drought stress tolerance in plants[25], fruit development, and ripening[2629].

    In this study, RNA-seq data were obtained for the de novo assembly and characterization of the S. thurberi fruit peel transcriptome. As a first approach, three transcripts, StCYP77A, StABCG11, and StGDSL1, tentatively involved in cuticle biosynthesis, were identified and quantified during sweet pitaya fruit development. Due to no gene expression analysis having been carried out yet for S. thurberi, stably expressed constitutive genes were identified for the first time.

    Sweet pitaya fruits (S. thurberi) without physical damage were hand harvested from plants in a native conditions field located at Carbó, Sonora, México. They were collocated in a cooler containing dry ice and transported immediately to the laboratory. The superficial part of the peels (~1 mm deep) was removed carefully from the fruits using a scalpel. Peel samples from three fruits were pooled according to their tentative stage of development defined by their visual characteristics, frozen in liquid nitrogen, and pulverized to create a single biological replicate. Four samples belonging to four different plants were analyzed. All fruits harvested were close to the ripening stage. Samples named M1 and M2 were turning from green to ripe [~35−40 Days After Flowering (DAF)], whereas samples M3 and M4 were turning from ripe to overripe (~40−45 DAF).

    Total RNA was isolated from the peels through the Hot Borate method[30]. The concentration and purity of RNA were determined in a spectrophotometer Nanodrop 2000 (Thermo Fisher) by measuring the 260/280 and 260/230 absorbance ratios. RNA integrity was evaluated through electrophoresis in agarose gel 1% and a Bioanalyzer 2100 (Agilent). Pure RNA was sequenced in the paired-end mode in an Illumina NextSeq 500 platform at the University of Arizona Genetics Core Facility. Four RNA-seq libraries, each of them from each sample, were obtained, which include a total of 288,199,704 short reads with a length of 150 base pairs (bp). The resulting sequence data can be accessed at the Sequence Read Archive (SRA) repository of the NCBI through the BioProject ID PRJNA1030439. Libraries are named corresponding to the names of samples M1, M2, M3, and M4.

    FastQC software (www.bioinformatics.babraham.ac.uk/projects/fastqc) was used for short reads quality analysis. Short reads with poor quality were trimmed or eliminated by Trimmomatic (www.usadellab.org/cms/?page=trimmomatic) with a trailing and leading of 25, a sliding window of 4:25, and a minimum read length of 80 bp. A total of 243,194,888 reads with at least a 25 quality score on the Phred scale were used to carry out the de novo assembly by Trinity (https://github.com/trinityrnaseq/trinityrnaseq/wiki) with the following parameters: minimal k-mer coverage of 1, normalization of 50, and minimal transcript length of 200 bp.

    Removal of contaminating sequences and ribosomal RNA (rRNA) was carried out through SeqClean. To remove redundancy, transcripts with equal or more than 90% of identity were merged through CD-hit (www.bioinformatics.org/cd-hit/). Alignment and quantification in terms of transcripts per million (TPM) were carried out through Bowtie (https://bowtie-bio.sourceforge.net/index.shtml) and RSEM (https://github.com/deweylab/RSEM), respectively. Transcripts showing a low expression (TPM < 0.01) were discarded. Assembly quality was evaluated by calculating the parameters N50 value, mean transcript length, TransRate score, and completeness. The statistics of the transcriptome were determined by TrinityStats and TransRate (https://hibberdlab.com/transrate/). The transcriptome completeness was determined through a BLASTn alignment (E value < 1 × 10−3) by BUSCO (https://busco.ezlab.org/) against the database of conserved orthologous genes from Embryophyte.

    To predict the proteins tentatively coded in the S. thurberi transcriptome, the best homology match of the assembled transcripts was found by alignment to the Swiss-Prot, RefSeq, nr-NCBI, PlantTFDB, iTAK, TAIR, and ITAG databases using the BLAST algorithm with an E value threshold of 1 × 10−10 for the nr-NCBI database and of 1 × 10−5 for the others[3134]. An additional alignment was carried out to the protein databases of commercial fruits Persea americana, Prunus persica, Fragaria vesca, Citrus cinensis, and Vitis vinifera to proteins of the cactus Opuntia streptacantha, and the transcriptomes of the cactus Hylocereus polyrhizus, Pachycereus pringlei, and Selenicereus undatus. The list of all databases and the database websites of commercial fruits and cactus are provided in Supplementary Tables S1 & S2. The open reading frame (ORF) of the transcripts and the protein sequences tentative coded from the sweet pitaya transcriptome was predicted by TransDecoder (https://github.com/TransDecoder/TransDecoder/wiki), considering a minimal ORF length of 75 amino acids (aa). The search for protein domains was carried out by the InterPro database (www.ebi.ac.uk/interpro). Functional categorization was carried out by Blast2GO based on GO terms and KEGG metabolic pathways[35].

    LncRNA were identified based on the methods reported in previous studies[25,29,36]. Transcripts without homology to any protein from Swiss-Prot, RefSeq, nr-NCBI, PlantTFDB, iTAK, TAIR, ITAG, P. americana, P. persica, F. vesca, C. cinensis, V. vinifera, and O. streptacantha databases, without a predicted ORF longer than 75 aa, and without protein domains in the InterPro database were selected to identify tentative lncRNA.

    Transcripts coding for signal peptide or transmembrane helices were identified by SignalP (https://services.healthtech.dtu.dk/services/SignalP-6.0/) and TMHMM (https://services.healthtech.dtu.dk/services/TMHMM-2.0/), respectively, and discarded. Further, transcripts corresponding to other non-coding RNAs (ribosomal RNA and transfer RNA) were identified through Infernal by using the Rfam database[37] and discarded. The remaining transcripts were analyzed by CPC[38], and CPC2[39] to calculate their coding potential. Transcripts with a coding potential score lower than −1 for CPC and a coding probability lower than 0.1 for CPC2 were considered lncRNA. To characterize the identified lncRNA, the length and abundance of coding and lncRNA were calculated. Bowtie and RSEM were used to align and quantify raw counts, respectively. The edgeR package[40] was used to normalize raw count data in terms of counts per million (CPM) for both coding and lncRNA.

    To obtain the transcript's expression, the aligning of short reads and quantifying of transcripts were carried out through Bowtie and RSEM software, respectively. A differential expression analysis was carried out between the four libraries by edgeR package in R Studio. Only the transcripts with a count equal to or higher than 0.5 in at least one sample were retained for the analysis. Transcripts with log2 Fold Change (log2FC) between +1 and −1 and with a False Discovery Rate (FDR) lower than 0.05 were taken as not differentially expressed (NDE).

    For the identification of the tentative reference genes two strategies were carried out as described below: i) The NDE transcripts were aligned by BLASTn (E value < 1 × 10−5) to 43 constitutive genes previously reported in fruits from the cactus H. polyrhizus, S. monacanthus, and S. undatus[4143] to identify possible homologous constitutive genes in S. thurberi. Then, the homologous transcripts with the minimal coefficient of variation (CV) were selected; ii) For all the NDE transcripts, the percentile 95 value of the mean CPM and the percentile 5 value of the CV were used as filters to recover the most stably expressed transcripts, based on previous studies[44]. Finally, transcripts to be tested by quantitative reverse transcription polymerase chain reaction (qRT-PCR) were selected based on their homology and tentative biological function.

    The fruit harvesting was carried out as described above. Sweet pitaya fruit takes about 43 d to ripen, therefore, open flowers were tagged, and fruits with 10, 20, 30, 35, and 40 DAF were collected to cover the pitaya fruit development process (Supplementary Fig. S1). The superficial part of the peels (~1 mm deep) was removed carefully from the fruits using a scalpel. Peel samples from three fruits were pooled according to their stage of development defined by their DAF, frozen in liquid nitrogen, and pulverized to create a single biological replicate. One biological replicate consisted of peels from three fruits belonging to the same plant. Two to three biological replicates were evaluated for each developmental stage. Two technical replicates were analyzed for each biological replicate. RNA extraction, quantification, RNA purity, and RNA integrity analysis were carried out as described above.

    cDNA was synthesized from 100 ng of RNA by QuantiTect Reverse Transcription Kit (QIAGEN). Primers were designed using the PrimerQuest™, UNAFold, and OligoAnalyzer™ tools from Integrated DNA Technologies (www.idtdna.com/pages) and following the method proposed by Thornton & Basu[45]. Transcripts quantification was carried out in a QIAquant 96 5 plex according to the PowerUp™ SYBR™ Green Master Mix protocol (Applied Biosystems), with a first denaturation step for 2 min at 95 °C, followed by 40 cycles of denaturation step at 95 °C for 15 s, annealing and extension steps for 30 s at 60 °C.

    The Cycle threshold (Ct) values obtained from the qRT-PCR were analyzed through the algorithms BestKeeper, geNorm, NormFinder, and the delta Ct method[46]. RefFinder (www.ciidirsinaloa.com.mx/RefFinder-master/) was used to integrate the stability results and to find the most stable expressed transcripts in sweet pitaya fruit peel during development. The pairwise variation value (Vn/Vn + 1) was calculated through the geNorm algorithm in R Studio software[47].

    An alignment of 17 reported cuticle biosynthesis genes from model plants were carried out by BLASTx against the predicted proteins from sweet pitaya. Two additional alignments of 17 charaterized cuticle biosynthesis proteins from model plants against the transcripts and predicted proteins of sweet pitaya were carried out by tBLASTn and BLASTp, respectively. An E value threshold of 1 × 10−5 was used, and the unique best hits were recovered for all three alignments. The sequences of the 17 characterized cuticle biosynthesis genes and proteins from model plants are showed in Supplementary Table S3. The specific parameters and the unique best hits for all the alignments carried out are shown in Supplementary Tables S4S8.

    Cuticle biosynthesis-related transcripts tentatively coding for a cytochrome p450 family 77 subfamily A (CYP77A), a Gly-Asp-Ser-Leu motif lipase/esterase 1 (GDSL1), and an ATP binding cassette transporter subfamily G member 11 (ABCG11) were identified by best bi-directional hit according to the functional annotation described above. Protein-conserved domains, signal peptide, and transmembrane helix were predicted through InterProScan, SignalP 6.0, and TMHMM, respectively. Alignment of the protein sequences to tentative orthologous of other plant species was carried out by the MUSCLE algorithm[48]. A neighbor-joining (NJ) phylogenetic tree with a bootstrap of 1,000 replications was constructed by MEGA11[49].

    Fruit sampling, primer design, RNA extraction, cDNA synthesis, and transcript quantification were performed as described above. Relative expression was calculated according to the 2−ΔΔCᴛ method[50]. The sample corresponding to 10 DAF was used as the calibrator. The transcripts StEF1a, StTUA, StUBQ3, and StEF1a + StTUA were used as normalizer genes.

    Normality was assessed according to the Shapiro-Wilk test. Significant differences in the expression of the cuticle biosynthesis-related transcripts between fruit developmental stages were determined by one-way ANOVA based on a completely randomized sampling design and a Tukey honestly significant difference (HSD) test, considering a p-value < 0.05 as significant. Statistical analysis was carried out through the stats package in R Studio.

    RNA was extracted from the peels of ripe sweet pitaya fruits (S. thurberi) from plants located in the Sonoran Desert, Mexico. Four cDNA libraries were sequenced in an Illumina NextSeq 500 platform at the University of Arizona Genetics Core Facility. A total of 288,199,704 reads with 150 base pairs (bp) in length were sequenced in paired-end mode. After trimming, 243,194,888 (84.38%) cleaned short reads with at least 29 mean quality scores per read in the Phred scale and between 80 to 150 bp in length were obtained to carry out the assembly. After removing contaminating sequences, redundancy, and low-expressed transcripts, the assembly included 174,449 transcripts with an N50 value of 2,110 bp. Table 1 shows the different quality variables of the S. thurberi fruit peel transcriptome. BUSCO score showed that 85.4% are completed transcripts, although out of these, 37.2% were found to be duplicated. The resulting sequence data can be accessed at the SRA repository of the NCBI through the BioProject ID PRJNA1030439.

    Table 1.  Quality metrics of the Stenocereus thurberi fruit peel transcriptome.
    Metric Data
    Total transcripts 174,449
    N50 2,110
    Smallest transcript length (bp) 200
    Largest transcript length (bp) 19,114
    Mean transcript length (bp) 1,198.69
    GC (%) 41.33
    Total assembled bases 209,110,524
    TransRate score 0.05
    BUSCO score (%) C: 85.38 (S:48.22, D:37.16),
    F: 10.69, M: 3.93.
    Values were calculated through the TrinityStats function of Trinity and TransRate software. Completeness analysis was carried out through BUSCO by aligning the transcriptome to the Embryophyte database through BLAST with an E value threshold of 1 × 10−3. Complete (C), single (S), duplicated (D), fragmented (F), missing (M).
     | Show Table
    DownLoad: CSV

    A summary of the homology search in the main public protein database for the S. thurberi transcriptome is shown in Supplementary Table S1. From these databases, the higher homologous transcripts were found in RefSeq with 93,993 (53.87 %). Based on the E value distribution, for 41,685 (44%) and 68,853 (49%) of the hits, it was found a strong homology (E value lower than 1 × 10−50) to proteins in the Swiss-Prot and RefSeq databases, respectively (Supplementary Fig. S2a & b). On the other hand, 56,539 (52.34%) and 99,599 (71.11%) of the matches showed a percentage of identity higher than 60% in the Swiss-Prot and RefSeq databases, respectively (Supplementary Fig. S2c & d).

    Figure 1 shows the homology between transcripts from S. thurberi and proteins of commercial fruits, as well as proteins and transcripts of cacti. Transcripts from S. thurberi homologous to proteins from fruits of commercial interest avocado (P. americana), peach (P. persica), strawberry (F. vesca), orange (C. sinensis), and grapefruit (V. vinifera) ranged from 77,285 (44.30%) to 85,421 (48.96%), with 70,802 transcripts homologous to all the five fruit protein databases (Fig. 1a).

    Transcripts homologous to transcripts or proteins from the cactus dragon fruit (H. polyrhizus), prickly pear cactus (O. streptacantha), Mexican giant cardon (P. pringlei), and pitahaya (S. undatus) ranged from 76,238 (43.70%) to 114,933 (65.88%), with 64,009 transcripts homologous to all the four cactus databases (Fig. 1b). Further, out of the total of transcripts, 44,040 transcripts (25.25%) showed homology only to sequences from cactus, but not for model plants Arabidopsis, tomato, or the commercial fruits included in this study (Fig. 1c).

    Figure 1.  Venn diagram of the homology search results against model plants databases, commercial fruits, and cactus. The number in the diagram corresponds to the number of transcripts from S. thurberi homologous to sequences from that plant species. (a) Homologous to sequences from Fragaria vesca (Fa), Persea americana (Pa), Prunus persica (Pp), Vitis vinifera (Vv), and Citrus sinensis (Cs). (b) Homologous to sequences from Opuntia streptacantha (Of), Selenicereus undatus (Su), Hylocereus polyrhizus (Hp), and Pachycereus pringlei (Pap). (c) Homologous to sequences from Solanum lycopersicum (Sl), Arabidopsis thaliana (At), from the commercial fruits (Fa, Pa, Pp, Vv, and Cs), or the cactus included in this study (Of, Su, Hp, and Pap). Homologous searching was carried out by BLAST alignment (E value < 1 × 10−5). The Venn diagrams were drawn by ggVennDiagram in R Studio.

    A total of 45,970 (26.35%), 58,704 (33.65%), and 48,186 (27.65%) transcripts showed homology to transcription factors, transcriptional regulators, and protein kinases in the PlantTFDB, iTAK-TR, and iTAK-PK databases, respectively (Supplementary Tables S1, S9S11). For the PlantTFDB, the homologous transcripts belong to 57 transcriptional factors (TF) families (Fig. 2 & Supplementary Table S9), from which, the most frequent were the basic-helix-loop-helix (bHLH), myeloblastosis-related (MYB-related), NAM, ATAF, and CUC (NAC), ethylene responsive factor (ERF), and the WRKY domain families (WRKY) (Fig. 2).

    Figure 2.  Transcription factor (TF) families distribution of S. thurberi fruit peel transcriptome. The X-axis indicates the number of transcripts with hits to each TF family. Alignment to the PlantTFDB database by BLASTx was carried out with an E value threshold of 1 × 10−5. The bar graph was drawn by ggplot2 in R Studio.

    Based on the homology found and the functional domain searches, gene ontology terms (GO) were assigned to 68,559 transcripts (Supplementary Table S12). Figure 3 shows the top 20 GO terms assigned to the S. thurberi transcriptome, corresponding to the Biological Processes (BP) and Molecular Function (MF) categories. For BP, organic substance metabolic processes, primary metabolic processes, and cellular metabolic processes showed a higher number of transcripts (Supplementary Table S13). Further, for MF, organic cyclic compound binding, heterocyclic compound binding, and ion binding were the processes with the higher number of transcripts. S. thurberi transcripts were classified into 142 metabolic pathways from the KEGG database (Supplementary Table S14). The pathways with the higher number of transcripts recorded were pyruvate metabolism, glycerophospholipid metabolism, glycolysis/gluconeogenesis, and citrate cycle. Further, among the top 20 KEEG pathways, the cutin, suberin, and wax biosynthesis include more than 30 transcripts (Fig. 4).

    Figure 3.  Top 20 Gene Ontology (GO) terms assigned to the S. thurberi fruit peel transcriptome. Bars indicate the number of transcripts assigned to each GO term. Assignment of GO terms was carried out by Blast2GO with default parameters. BP and MF mean Biological Processes and Molecular Functions GO categories, respectively. The graph was drawn by ggplot2 in R Studio.
    Figure 4.  Top 20 KEGG metabolic pathways distribution in the S. thurberi fruit peel transcriptome. Bars indicate the number of transcripts assigned to each KEGG pathway. Assignment of KEGG pathways was carried out in the Blast2GO suite. The bar graph was drawn by ggplot2 in R Studio.

    Out of the total of transcripts, 43,391 (24.87%) were classified as lncRNA (Supplementary Tables S15 & S16). Figure 5 shows a comparison of the length (Fig. 5a) and expression (Fig. 5b) of lncRNA and coding RNA. Both length and expression values were higher in coding RNA than in lncRNA. In general, coding RNA ranged from 201 to 18,629 bp with a mean length of 1,507.18, whereas lncRNA ranged from 200 to 5,198 bp with a mean length of 481.51 (Fig. 5a). The higher expression values recorded from coding RNA and lncRNA were 12.83 and 9.45 log2(CPM), respectively (Fig. 5b).

    Figure 5.  Comparison of coding RNA and long non-coding RNA (lncRNA) from S. thurberi transcriptome. (a) Box plot of transcript length distribution. The Y-axis indicates the length of each transcript in base pairs. (b) Box plot of expression levels. The Y-axis indicates the log2 of the count per million of reads (log2(CPM)) recorded for each transcript. Expression levels were calculated by the edgeR package in R studio. (a), (b) The lines inside the boxes indicate the median. The higher and lower box limits represent the 75th and 25th percentiles, respectively. The box plots were drawn by ggplot2 in R Studio.

    To identify the transcripts without significant changes in expression between the four RNA-seq libraries, a differential expression analysis was carried out. Of the total of transcripts, 4,980 were not differentially expressed (NDE) at least in one paired comparison between the libraries (Supplementary Tables S17S20). Mean counts per million of reads (CPM) and coefficient of variation (CV)[44] were calculated for these NDE transcripts. Transcripts with a CV value lower than 0.113, corresponding with the percentile 5 of the CV, and a mean CPM higher than 1,138.06, corresponding with the percentile 95 of the mean CPM were used as filters to identify the most stably expressed transcripts (Supplementary Table S21). Based on its homology and its tentative biological function, five transcripts were selected to be tested as tentative reference genes. Besides, three NDE transcripts homologous to previously identified stable expressed reference genes in other species of cactus fruit[4143] were selected (Supplementary Table S22). Homology metrics for the eight tentative reference genes selected are shown in Supplementary Table S23. The primer sequences used to amplify the transcripts by qRT-PCR and their nucleotide sequence are shown in Supplementary Tables S24 & S25, respectively.

    The amplification specificity of the eight candidate reference genes determined by melting curves analysis is shown in Supplementary Fig. S3. For the eight tentative reference transcripts selected, the cycle threshold (Ct) values were recorded during sweet pitaya fruit development by qRT-PCR (Supplementary Table S26). The Ct values obtained ranged from 16.85 to 30.26 (Fig. 6a). Plastidic ATP/ADP-transporter (StTLC1) showed the highest Ct values with a mean of 27.34 (Supplementary Table S26). Polyubiquitin 3 (StUBQ3) showed the lowest Ct values in all five sweet pitaya fruit developmental stages (Fig. 6a).

    Figure 6.  Expression stability analysis of tentative reference genes. (a) Box plot of cycle threshold (Ct) distribution of candidate reference genes during sweet pitaya fruit development (10, 20, 30, 35, and 40 d after flowering). The black line inside the box indicates the median. The higher and lower box limits represent the 75th and 25th percentiles, respectively. (b) Bar chart of the geometric mean (geomean) of ranking values calculated by RefFinder for each tentative reference gene (X-axis). The lowest values indicate the best reference genes. (c) Bar chart of the pairwise variation analysis and determination of the optimal number of reference genes by the geNorm algorithm. A pairwise variation value lower than 0.15 indicates that the use of Vn/Vn + 1 reference genes is reliable for the accurate normalization of qRT-PCR data. The Ct data used in the analysis were calculated by qRT-PCR in a QIAquant 96 5 plex (QIAGEN) according to the manufacturer's protocol. The box plot and the bar graphs were drawn by ggplot2 and Excel programs, respectively. Abbreviations: Actin 7 (StACT7), alpha-tubulin (StTUA), elongation factor 1-alpha (StEF1a), COP1-interactive protein 1 (StCIP1), plasma membrane ATPase 4 (StPMA4), BEL1-like homeodomain protein 1 (StBLH1), polyubiquitin 3 (StUBQ3), and plastidic ATP/ADP-transporter (StTLC1).

    The best stability values calculated by NormFinder were 0.45, 0.51, 0.97, and 0.99, corresponding to the transcripts elongation factor 1-alpha (StEF1a), alpha-tubulin (StTUA), plastidic ATP/ADP-transporter (StTLC1), and actin 7 (StACT7), respectively (Supplementary Table S27). For BestKeeper, the most stable expressed transcripts were StUBQ3, StTUA, and StEF1a, with values of 0.72, 0.75, and 0.87, respectively. In the case of the delta Ct method[51], the transcripts StEF1a, StTUA, and StTLC1 showed the best stability.

    According to geNorm analysis, the most stable expressed transcripts were StTUA, StEF1a, StUBQ3, and StACT7, with values of 0.74, 0.74, 0.82, and 0.96, respectively. All the pairwise variation values (Vn/Vn + 1) were lower than 0.15, ranging from 0.019 for V2/V3 to 0.01 for V6/V7 (Fig. 6c). The V value of 0.019 obtained for V2/V3 indicates that the use of the best two reference genes StTUA and StEF1a is reliable enough for the accurate normalization of qRT-PCR data, therefore no third reference gene is required[47]. Except for BestKeeper analysis, StEF1a and StTUA were the most stable transcripts for all of the methods carried out in this study (Supplementary Table S27). The comprehensive ranking analysis indicates that StEF1a, followed by StTUA and StUBQ3, are the most stable expressed genes and are stable enough to be used as reference genes in qRT-PCR analysis during sweet pitaya fruit development (Fig. 6b).

    Three cuticle biosynthesis-related transcripts TRINITY_DN17030_c0_g1_i2, TRINITY_DN15394_c0_g1_i1, and TRINITY_DN23528_c1_g1_i1 tentatively coding for the enzymes cytochrome p450 family 77 subfamily A (CYP77A), Gly-Asp-Ser-Leu motif lipase/esterase 1 (GDSL1), and an ATP binding cassette transporter subfamily G member 11 (ABCG11/WBC11), respectively, were identified and quantified. The nucleotide sequence and predicted amino acid sequences of the three transcripts are shown in Supplementary File 1. The best homology match for StCYP77A (TRINITY_DN17030_c0_g1_i2) was for AtCYP77A4 (AT5G04660) from Arabidopsis and SmCYP77A2 (P37124) from eggplant (Solanum melongena) in the TAIR and Swiss-Prot databases, respectively (Supplementary Table S23).

    TransDecoder, InterPro, and TMHMM analysis showed that StCYP77A codes a polypeptide of 518 amino acids (aa) in length that comprises a cytochrome P450 E-class domain (IPR002401) and a transmembrane region (residues 10 to 32). The phylogenetic tree constructed showed that StCYP77A is grouped in a cluster with all the CYP77A2 proteins included in this analysis, being closer to CYP77A2 (XP_010694692) from B. vulgaris and Cgig2_012892 (KAJ8441854) from Carnegiea gigantean (Supplementary Fig. S4).

    StGDSL1 (TRINITY_DN15394_c0_g1_i1) alignment showed that it is homologous to a GDSL esterase/lipase from Arabidopsis (Q9LU14) and tomato (Solyc03g121180) (Supplementary Table S23). TransDecoder, InterPro, and SignalP analysis showed that StGDSL1 codes a polypeptide of 354 aa in length that comprises a GDSL lipase/esterase domain IPR001087 and a signal peptide with a cleavage site between position 25 and 26 (Supplementary Fig. S5).

    Supplementary Figure S6 shows the analysis carried out on the predicted amino acid sequence of StABCG11 (TRINITY_DN23528_c1_g1_i1). The phylogenetic tree constructed shows three clades corresponding to the ABCG13, ABCG12, and ABCG11 protein classes with bootstrap support ranging from 40% to 100% (Supplementary Fig. S6a). StABCG11 is grouped with all the ABCG11 transporters included in this study in a well-separated clade, being closely related to its tentative ortholog from C. gigantean Cgig2_004465 (KAJ8441854). InterPro and TMHMM results showed that the StABCG11 sequence contains an ABC-2 type transporter transmembrane domain (IPR013525; PF01061.27) with six transmembrane helices (Supplementary Fig. S6b).

    The predicted protein sequence of StABCG11 is 710 aa in length, holding the ATP binding domain (IPR003439; PF00005.30) and the P-loop containing nucleoside triphosphate hydrolase domain (IPR043926; PF19055.3) of the ABC transporters of the G family. Multiple sequence alignment shows that the Walker A and B motif sequence and the ABC signature[15] are conserved between the ABCG11 transporters from Arabidopsis, tomato, S. thurberi, and C. gigantean (Supplementary Fig. S6c).

    According to the results of the expression stability analysis (Fig. 6), four normalization strategies were tested to quantify the three cuticle biosynthesis-related transcripts during sweet pitaya fruit development. The four strategies consist of normalizing by StEF1a, StTUA, StUBQ3, or StEF1a+StTUA. Primer sequences used to quantify the transcripts StCYP77A (TRINITY_DN17030_c0_g1_i2), StGDSL1 (TRINITY_DN15394_c0_g1_i1), and StABCG11 (TRINITY_DN23528_c1_g1_i1) by qRT-PCR during sweet pitaya fruit development are shown in Supplementary Table S24.

    The three cuticle biosynthesis-related transcripts showed differences in expression during sweet pitaya fruit development (Supplementary Table S28). The same expression pattern was recorded for the three cuticle biosynthesis transcripts when normalization was carried out by StEF1a, StTUA, StUBQ3, or StEF1a + StTUA (Fig. 7). A higher expression of StCYP77A and StGDSL1 are shown at the 10 and 20 DAF, showing a decrease at 30, 35, and 40 DAF. StABCG11 showed a similar behavior, with a higher expression at 10 and 20 DAF and a reduction at 30 and 35 DAF. Nevertheless, unlike StCYP77A and StGDSL1, a significant increase at 40 DAF, reaching the same expression as compared with 10 DAF, is shown for StABCG11 (Fig. 7).

    Figure 7.  Expression analysis of cuticle biosynthesis-related transcripts StCYP77A, StGDSL1, and StABCG11 during sweet pitaya (Stenocereus thurberi) fruit development. Relative expression was calculated through the 2−ΔΔCᴛ method using elongation factor 1-alpha (StEF1a), alpha-tubulin (StTUA), polyubiquitin 3 (StUBQ3), or StEF1a + StTUA as normalizing genes at 10, 20, 30, 35, and 40 d after flowering (DAF). The Y-axis and error bars represent the mean of the relative expression ± standard error (n = 4−6) for each developmental stage in DAF. The Ct data for the analysis was recorded by qRT-PCR in a QIAquant 96 5 plex (QIAGEN) according to the manufacturer's protocol. The graph line was drawn by ggplot2 in R Studio. Abbreviations: cytochrome p450 family 77 subfamily A (StCYP77A), Gly-Asp-Ser-Leu motif lipase/esterase 1 (StGDSL1), and ATP binding cassette transporter subfamily G member 11 (StABCG11).

    Characteristics of a well-assembled transcriptome include an N50 value closer to 2,000 bp, a high percentage of conserved transcripts completely assembled (> 80%), and a high proportion of reads mapping back to the assembled transcripts[52]. In the present study, the first collection of 174,449 transcripts from S. thurberi fruit peel are reported. The generated transcriptome showed an N50 value of 2,110 bp, a TransRate score of 0.05, and a GC percentage of 41.33 (Table 1), similar to that reported for other de novo plant transcriptome assemblies[53]. According to BUSCO, 85.4% of the orthologous genes from the Embryophyta databases completely matched the S. thurberi transcriptome, and only 3.9% were missing (Table 1). These results show that the S. thurberi transcriptome generated is not fragmented, and it is helpful in predicting the sequence of almost all the transcripts expressed in sweet pitaya fruit peel[24].

    The percentage of transcripts homologous found, E values, and identity distribution (Supplementary Tables S1 & S2; Supplementary Fig. S2) were similar to that reported in the de novo transcriptome assembly for non-model plants and other cactus fruits[4143,54] and further suggests that the transcriptome assembled of S. thurberi peel is robust[52]. Of the total of transcripts, 70,802 were common to all the five commercial fruit protein databases included in this study, which is helpful for the search for conserved orthologous involved in fruit development and ripening (Fig. 2a). A total of 34,513 transcripts (20%) show homology only to sequences in the cactus's databases, but not in the others (Supplementary Tables S1 & S2; Fig. 1c). This could suggest that a significant conservation of sequences among plants of the Cactaceae family exists which most likely are to have a function in this species adaptation to desert ecosystems.

    To infer the biological functionality represented by the S. thurberi fruit peel transcriptome, gene ontology (GO) terms and KEGG pathways were assigned. Of the main metabolic pathways assigned, 'glycerolipid metabolism' and 'cutin, suberine, and wax biosynthesis' suggests an active cuticle biosynthesis in pitaya fruit peel (Fig. 4). In agreement with the above, the main GO terms assigned for the molecular function (MF) category were 'organic cyclic compound binding', 'transmembrane transporter activity', and 'lipid binding' (Fig. 3). For the biological processes (BP) category, the critical GO terms for the present research are 'cellular response to stimulus', 'response to stress', 'anatomical structure development', and 'transmembrane transport', which could suggest the active development of the fruit epidermis and cuticle biosynthesis for protection to stress.

    The most frequent transcription factors (TF) families found in S. thurberi transcriptome were NAC, WRKY, bHLH, ERF, and MYB-related (Fig. 2), which had been reported to play a function in the tolerance to abiotic stress in plants[55,56]. Although the role of NAC, WRKY, bHLH, ERF, and MYB TF in improving drought tolerance in relevant crop plants has been widely documented[57,58], their contribution to the adaptation of cactus to arid ecosystems has not yet been elucidated and further experimental pieces of evidence are needed.

    It has been reported that the heterologous expression of ERF TF from Medicago truncatula induces drought tolerance and cuticle wax biosynthesis in Arabidopsis leaf[59]. In tomato fruits, the gene SlMIXTA-like which encodes a MYB transcription factor avoids water loss through the positive regulation of genes related to the biosynthesis and transport of cuticle compounds[22]. Despite the relevant role of cuticles in maintaining cactus physiology in desert environments, experimental evidence showing the role of the different TF-inducing cuticle biosynthesis has yet to be reported for cactus fruits.

    Out of the transcripts, 43,391 were classified as lncRNA (Supplementary Tables S15 & S16). This is the first report of lncRNA identification for the species S. thurberi. In fruits, 3,679 lncRNA has been identified from tomato[26], 3,330 from peach (P. persica)[29], 3,857 from melon (Cucumis melo)[28], 2,505 from hot pepper (Capsicum annuum)[27], and 3,194 from pomegranate (Punica granatum)[36]. Despite the stringent criteria to classify the lncRNA of sweet pitaya fruit (S. thurberi), a higher number of lncRNAs are shown when compared with previous reports. This finding is most likely due to the higher level of redundancy found during the transcriptome analysis. To reduce this redundancy, further efforts to achieve the complete genome assembly of S. thurberi are needed.

    Previous studies showed that lncRNA is shorter and has lower expression levels than coding RNA[6062]. In agreement with those findings, both the length and expression values of lncRNA from S. thurberi were lower than coding RNA (Fig. 5). It has been suggested that lncRNA could be involved in the biosynthesis of cuticle components in cabbage[61] and pomegranate[36] and that they could be involved in the tolerance to water deficit through the regulation of cuticle biosynthesis in wild banana[60]. Nevertheless, the molecular mechanism by which lncRNA may regulate the cuticle biosynthesis in S. thurberi fruits has not yet been elucidated.

    A relatively constant level of expression characterizes housekeeping genes because they are involved in essential cellular functions. These genes are not induced under specific conditions such as biotic or abiotic stress. Because of this, they are very useful as internal reference genes for qRT-PCR data normalization[63]. Nevertheless, their expression could change depending on plant species, developmental stages, and experimental conditions[64]. Reliable reference genes for a specific experiment in a given species must be identified to carry out an accurate qRT-PCR data normalization[63]. An initial screening of the transcript expression pattern through RNA-seq improves the identification of stably expressed transcripts by qRT-PCR[44,64].

    Identification of stable expressed reference transcripts during fruit development has been carried out in blueberry (Vaccinium bracteatum)[65], kiwifruit (Actinidia chinensis)[66], peach (P. persica)[67], apple (Malus domestica)[68], and soursop (Annona muricata)[69]. These studies include the expression stability analysis through geNorm, NormFinder, and BestKeeper algorithms[68,69], some of which are supported in RNA-seq data[65,66]. Improvement of expression stability analysis by RNA-seq had led to the identification of non-previously reported reference genes with a more stable expression during fruit development than commonly known housekeeping genes in grapevine (V. vinifera)[44], pear (Pyrus pyrifolia and P. calleryana)[64], and pepper (C. annuum)[70].

    For fruits of the Cactaceae family, only a few studies identifying reliable reference genes have been reported[4143]. Mainly because gene expression analysis has not been carried out previously for sweet pitaya (S. thurberi), the RNA-seq data generated in this work along with geNorm, NormFinder, BestKeeper, and RefFinder algorithms were used to identify reliable reference genes. The comprehensive ranking analysis showed that out of the eight candidate genes tested, StEF1a followed by StTUA and StUBQ3 were the most stable (Fig. 6b). All the pairwise variation values (Vn/Vn + 1) were lower than 0.15 (Fig. 6c), which indicates that StEF1a, StTUA, and StUBQ3 alone or the use of StEF1a and StTUA together are reliable enough to normalize the gene expression data generated by qRT-PCR.

    The genes StEF1a, StTUA, and StUBQ3 are homologous to transcripts found in the cactus species known as dragonfruit (Hylocereus monacanthus and H. undatus)[41], which have been tested as tentative reference genes during fruit development. EF1a has been proposed as a reliable reference gene in the analysis of changes in gene expression of dragon fruit (H. monacanthus and H. undatus)[41], peach (P. persica)[67], apple (M. domestica)[68], and soursop (A. muricata)[69]. According to the expression stability analysis carried out in the present study (Fig. 6) four normalization strategies were designed. The same gene expression pattern was recorded for the three target transcripts evaluated when normalization was carried out by the genes StEF1a, StTUA, StUBQ3, or StEF1a + StTUA (Fig. 7). Further, these data indicates that these reference genes are reliable enough to be used in qRT-PCR experiments during fruit development of S. thurberi.

    The plant cuticle is formed by two main layers: the cutin, composed mainly of mid-chain oxygenated LC fatty acids, and the cuticular wax, composed mainly of very long-chain (VLC) fatty acids, and their derivates VLC alkanes, VLC primary alcohols, VLC ketones, VLC aldehydes, and VLC esters[3]. In Arabidopsis CYP77A4 and CYP77A6 catalyze the synthesis of midchain epoxy and hydroxy ω-OH long-chain fatty acids, respectively[10,11], which are the main components of fleshy fruit cuticle[3].

    The functional domain search carried out in the present study showed that StCYP77A comprises a cytochrome P450 E-class domain (IPR002401) and a membrane-spanning region from residues 10 to 32 (Supplementary Fig. S4). This membrane-spanning region has been previously characterized in CYP77A enzymes from A. thaliana and Brassica napus[11,71]. It suggests that the protein coded by StCYP77A could catalyze the oxidation of fatty acids embedded in the endoplasmic reticulum membrane of the epidermal cells of S. thurberi fruit. Phylogenetic analysis showed that StCYP77A was closer to proteins from its phylogenetic-related species B. vulgaris (BvCYP772; XP_010694692) and C. gigantea (Cgig2_012892) (Supplementary Fig. S4). StCYP77A, BvCYP77A2, and Cgig2_012892 were closer to SlCYP77A2 and SmCYP77A2 than to CYP77A4 and CYP77A6 proteins, suggesting that StCYP77A (TRINITY_DN17030_c0_g1_i2) could correspond to a CYP77A2 protein.

    Five CYP77A are present in the Arabidopsis genome, named CYP77A4, CYP77A5, CYP77A6, CYP77A7, and CYP77A9, but their role in cuticle biosynthesis has only been reported for CYP77A4 and CYP77A6[72]. It has been suggested that CYP77A2 from eggplant (S. torvum) could contribute to the defense against fungal phytopathogen infection by the synthesis of specific compounds[13]. In pepper fruit (C. annuum), the expression pattern of CYP77A2 (A0A1U8GYB0) and ABCG11 (LOC107862760) suggests a role of CYP77A2 and ABCG11 in cutin biosynthesis at the early stages of pepper fruit development[14].

    In the case of the protein encoded by StGDSL1 (354 aa), the length found in this work is similar to the length of its homologous from Arabidopsis (AT3G16370) and tomato (Solyc03g121180) (Supplementary Fig. S5). A GDSL1 protein named CD1 polymerizes midchain oxygenated ω-OH long-chain fatty acids to form the cutin polyester in the extracellular space of tomato fruit peel[20,21]. It has been suggested that the 25-amino acid N-signal peptide found in StGDSL1 (Supplementary Fig. S5), previously reported in GDSL1 from Arabidopsis, B. napus, and tomato, plays a role during the protein exportation to the extracellular space[21,73].

    A higher expression of StCYP77A, StGDSL1, and StABCG11 is shown at the 10 and 20 DAF of sweet pitaya fruit development (Fig. 7), suggesting the active cuticle biosynthesis at the early stages of sweet pitaya fruit development. In agreement with that, two genes coding for GDSL lipase/hydrolases from tomato named SGN-U583101 and SGN-U579520 are highly expressed in the early stages and during the expansion stages of tomato fruit development, but their expression decreases in later stages[74]. It has been shown that the expression of GDSL genes, like CD1 from tomato, is higher in growing fruit[20,21]. Like tomato, the increase in expression of StCYP77A and StGDSL1 shown in pitaya fruit development could be due to an increase in cuticle deposition during the expansion of the fruit epidermis[20].

    The phylogenetic analysis, the functional domains, and the six transmembrane helices found in the StABCG11 predicted protein (Supplementary Fig. S6), suggests that it is an ABCG plasma membrane transporter of sweet pitaya[15]. Indeed, an increased expression of StABCG11 at 40 DAF was recorded in the present study (Fig. 7). Further, this data strongly suggests that it could be playing a relevant role in the transport of cuticle components at the beginning and during sweet pitaya fruit ripening.

    In Arabidopsis, ABCG11 (WBC11) exports cuticular wax and cutin compounds from the plasma membrane[15,75]. It has been reported that a high expression of the ABC plasma membrane transporter from mango MiWBC11 correlates with a higher cuticle deposition during fruit development[7]. The expression pattern for StABCG11, StCYP77A, and StGDSL1 suggests a role of StABCG11 as a cutin compound transporter in the earlier stages of sweet pitaya fruit development (Fig. 7). Further, its increase at 40 DAF suggests that it could be transporting cuticle compounds other than oxygenated long-chain fatty acids, or long-chain fatty acids that are not synthesized by StCYP77A and StGDSL1 in the later stages of fruit development.

    Like sweet pitaya, during sweet cherry fruit (Prunus avium) development, the expression of PaWCB11, homologous to AtABCG11 (AT1G17840), increases at the earlier stages of fruit development decreases at the intermediate stages, and increases again at the later stages[76]. PaWCB11 expression correlated with cuticle membrane deposition at the earlier and intermediate stages of sweet cherry fruit development but not at the later[76]. The increased expression of StABCG11 found in the present study could be due to the increased transport of cuticular wax compounds, such as VLC fatty acids and their derivates, in the later stages of sweet pitaya development[15,75].

    Cuticular waxes make up the smallest amount of the fruit cuticle. Even so, they mainly contribute to the impermeability of the fruit's epidermis[3]. An increase in the transport of cuticular waxes at the beginning of the ripening stage carried out by ABCG transporters could be due to a greater need to avoid water loss and to maintain an adequate amount of water during the ripening of the sweet pitaya fruit. Nevertheless, further expression analysis of cuticular wax biosynthesis-related genes, complemented with chemical composition analysis of cuticles could contribute to elucidating the molecular mechanism of cuticle biosynthesis in cacti and their physiological contribution during fruit development.

    In this study, the transcriptome of the sweet pitaya (S. thurberi) fruit peel was assembled for the first time. The reference genes found here are a helpful tool for further gene expression analysis in sweet pitaya fruit. Transcripts tentatively involved in cuticle compound biosynthesis and transport are reported for the first time in sweet pitaya. The results suggest a relevant role of cuticle compound biosynthesis and transport at the early and later stages of fruit development. The information generated will help to improve the elucidation of the molecular mechanism of cuticle biosynthesis in S. thurberi and other cactus species in the future. Understanding the cuticle's physiological function in the adaptation of the Cactaceae family to harsh environmental conditions could help design strategies to increase the resistance of other species to face the increase in water scarcity for agricultural production predicted for the following years.

    The authors confirm contribution to the paper as follows: study conception and design: Tiznado-Hernández ME, Tafolla-Arellano JC, García-Coronado H, Hernández-Oñate MÁ; data collection: Tiznado-Hernández ME, Tafolla-Arellano JC, García-Coronado H, Hernández-Oñate MÁ; analysis and interpretation of results: Tiznado-Hernández ME, García-Coronado H, Hernández-Oñate MÁ, Burgara-Estrella AJ; draft manuscript preparation: Tiznado-Hernández ME, García-Coronado H. All authors reviewed the results and approved the final version of the manuscript.

    All data generated or analyzed during this study are included in this published article and its supplementary information files. The sequence data can be accessed at the Sequence Read Archive (SRA) repository of the NCBI through the BioProject ID PRJNA1030439.

    The authors wish to acknowledge the financial support of Consejo Nacional de Humanidades, Ciencias y Tecnologías de México (CONAHCYT) through project number 579: Elucidación del Mecanismo Molecular de Biosíntesis de Cutícula Utilizando como Modelo Frutas Tropicales. We appreciate the University of Arizona Genetics Core and Illumina for providing reagents and equipment for library sequencing. The author, Heriberto García-Coronado (CVU 490952), thanks the CONAHCYT (acronym in Spanish) for the Ph.D. scholarship assigned (749341). The author, Heriberto García-Coronado, thanks Dr. Edmundo Domínguez-Rosas for the technical support in bioinformatics for identifying long non-coding RNA.

  • The authors declare that they have no conflict of interest.

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  • Cite this article

    Li Q, Li R, He F, Yang Z, Yu J. 2022. Growth and physiological effects of chitosan on heat tolerance in creeping bentgrass (Agrostis stolonifera). Grass Research 2:6 doi: 10.48130/GR-2022-0006
    Li Q, Li R, He F, Yang Z, Yu J. 2022. Growth and physiological effects of chitosan on heat tolerance in creeping bentgrass (Agrostis stolonifera). Grass Research 2:6 doi: 10.48130/GR-2022-0006

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Growth and physiological effects of chitosan on heat tolerance in creeping bentgrass (Agrostis stolonifera)

Grass Research  2 Article number: 6  (2022)  |  Cite this article

Abstract: High temperature is one of the major abiotic stresses limiting growth and development of cool-season grass species, but chitosan could effectively enhance heat tolerance and improve plant growth. The objective of this study was to determine the optimal concentration of chitosan that could alleviate heat stress in creeping bentgrass (Agrostis stolonifera) and investigate the effects of exogenous chitosan on photosynthesis and cell membrane stability under heat stress. Under heat stress (38/28 °C, day/night), different chitosan concentrations of 0, 50, 100 and 500 mg·L−1 were applied on the leaves of creeping bentgrass (cv. 'Penn-A4'). Foliar application of chitosan exhibited the positive effects on plant growth and the optimal concentration was 100 mg·L−1 which significantly improved turf quality, root length, root-shoot ratio as well as shoot and root biomass. Chitosan-treated plants subjected to high temperature stress had a lower decline in photosynthetic rate and photochemical efficiency as well as less increase in electrolyte leakage and malondialdehyde content. The results demonstrate that chitosan-improved heat tolerance as reflected by the superior growth performance of both shoot and root, photosynthesis and cell membrane stability in creeping bentgrass under heat stress.

    • Creeping bentgrass (Agrostis stolonifera) is one of the perennial cool-season turfgrass species with an optimal temperature growth temperature range of 18 to 24 °C[1]. It is not only extensively used on the greens of golf courses located in temperate and frigid areas but also utilized in transition and subtropical zones due to the superior characteristics of fine texture and high tolerance to low mowing height[2]. However, the atmospheric temperature often rises beyond the temperature threshold for optimal growth of creeping bentgrass, especially during the summer season[3]. High temperature is able to restrict the normal physiological metabolic activities leading to a decrease in photosynthesis[4], respiration[5] and antioxidant metabolism[6] in creeping bentgrass. Long-term heat stress caused turf quality decline, plant growth inhibition and leaf senescence[7]. In creeping bentgrass, green leaf number and growth height decreased gradually due to high temperature stress[8]. In addition, plant density, tiller density, number of roots and root fresh weight also reduced which was attributed to heat stress[9].

      Many approaches could be utilized to enhance heat tolerance in cool-season turfgrasses including breeding heat-tolerant cultivars[10], heat acclimation[11], cultivation and management measures[12] and chemical regulation[13]. Chemical regulation was reported to be one of the simplest and convenient methods to control plant growth and development by foliar application of exogenous substances to effectively mitigate heat stress injury in various plants, including perennial grass species. Among the majority of growth regulators, chitosan was previously documented to improve stress tolerance in many plants. Chitosan, a deacetylation product of chitin widely existing in plants, was natural, non-toxic and a biodegradable biopolymer extensively being used for food, medicine, agriculture and other fields[14, 15]. Chitosan emerged as being multifunctional as an elicitor in plants, which took part in seed germination, plant growth and development, biotic and abiotic stresses[1618]. On the one hand, chitosan significantly promoted shoot growth and root development in cordyline (Cordyline terminalis) seedlings and increased total biomass in milk thistle (Silybum marianum)[19, 20]. On the other hand, chitosan could also alleviate detrimental effects of various abiotic stresses, such as cold, drought and salinity stresses, through regulating the physiological responses of plants. Chitosan has been widely demonstrated to foster positive impacts on enhancing cold tolerance in tea plants (Camellia sinensis) as well as mitigating osmotic stress in rice (Oryza sativa) through increasing shoot fresh and dry weights[21, 22]. In white clover (Trifolium repens), chitosan-improved drought tolerance associated with changes in endogenous hormones and antioxidant systems[23]. In addition, exogenous chitosan significantly promoted root length and plant height of maize (Zea mays) seedlings under salt stress and reduced content of reactive oxygen species and malondialdehyde[24].

      Although previous studies have confirmed that chitosan is beneficial to stress tolerance in several plants, the function of chitosan in association with heat tolerance in perennial creeping bentgrass is not well understood. The objective of this study was to determine the optimal concentration of chitosan that could positively alleviate the adverse effects of heat stress and investigate the effects of exogenous chitosan on photosynthesis and cell membrane stability under heat stress. The results in this study would be useful to better understand the function of chitosan and provide effective approaches for mitigating high temperature stress in creeping bentgrass, especially in the summer season.

    • Creeping bentgrass seeds (A. stolonifera cv. 'Penn-A4') were planted in sand-filled polyvinyl chloride (PVC) pipes (10 cm in diameter and 25 cm in height). The plant material was grown in a greenhouse at 25/20 °C (day/night) for about 2 months. Plants were irrigated daily, maintained at a canopy height of approximately 4−5 cm by trimming every 2 d and fertilized once a week with water-soluble fertilizer (Scotts Miracle-Gro Company, USA). After establishment, plants were transferred to growth chambers (XBQH-1, Jinan Xubang, Jinan, Shandong Province, China) for acclimating 7 d before treatment initiation. The temperature of the growth chamber was set at 25/20 °C (day/night) with 14 h photoperiod and 60% relative humidity.

    • Plants were exposed to heat stress for 49 d in the growth chamber with treatment temperature of 38/28 °C (day/night) after one week of acclimation. This experiment was set as a completely randomized design with three biological replicates for each treatment. Different chitosan concentrations of 0, 50, 100 and 500 mg·L−1 as reported by Geng et al.[25] were respectively sprayed on the leaves at 0 d of treatment, and then applied every 7 d during the 49 d of heat stress. The treatment of deionized water without exogenous chitosan (0 mg·L−1) was set as the control. Chitosan (85% deacetylation degree) was dissolved in 1% (v/v) acetic acid and deionized water was added to achieve the desired concentrations. Creeping bentgrass was no longer trimmed but watered daily to ensure available water for plant growth during the experimental period. Plants were relocated randomly every day within the growth chamber to minimize the effects of environmental difference on treatments.

    • Turf quality was rated weekly on a scale from 1 to 9 based on texture, color, uniformity and density[26]. As a common index, turf quality represents overall turf performance with 1 being a completely dead turf and 9 being healthy turf with green and dense turf canopy. A rating of 6 indicates the minimal acceptable level of turf quality. The ratio of yellow leaves was calculated as the ratio of the number of yellow leaves with more than 50% of area being dry and showing chlorosis compared to the number of total leaves[27]. The number of yellow leaves and total leaves were counted on five labeled stolons once a week, respectively. Growth rate was calculated by measuring stolon length every week. Five stolons were randomly labeled in each PVC tube at 0 d of treatment and stolon length were measured using a ruler.

    • Root length was measured with a scale on the last day of treatment. Shoot and root were separated, put into the oven at 105 °C for 30 min, and then dried at 80 °C to a constant weight. Shoot biomass and root biomass were weighed respectively to calculate root-shoot ratio.

    • Following one week of acclimation to growth chamber conditions, plants were then exposed to four treatments in creeping bentgrass as follows: 1) control (control + H2O), plants sprayed with 10 mL deionized water under 25/20 °C (day/night); 2) only chitosan treatment (control + chitosan), plants with foliar application of 10 mL 100 mg·L-1 chitosan under non-stressed condition (25/20 °C, day/night); 3) high temperature treatment (H + H2O), plants were sprayed with 10 mL deionized water without the chitosan application under heat stress (38/28 °C, day/night) and 4) chitosan treatment and high temperature treatment (H + chitosan), plants were sprayed with 10 mL 100 mg·L−1 exogenous chitosan under heat stress (38/28 °C, day/night). Plants were treated with deionized water or chitosan every 7 d from the beginning at 0 d of treatment. The experiment was a completely randomized design with four biological replicates (four pots of plants) for each treatment.

    • Physiological indicators, including photosynthetic rate (Pn), photochemical efficiency (Fv/Fm), electrolyte leakage (EL) and malondialdehyde content (MDA) were measured at 0, 21 and 42 d of treatments. Leaf Fv/Fm, the ratio of variable fluorescence (Fv) to maximum fluorescence (Fm), was measured with a fluorescence induction monitor (OPTI-Sciences, Hudson, USA) after acclimating in dark for 30 min with leaf clips. Leaf Pn was detected using a LI-6400 portable photosynthesis system (LI-COR, Lincoln, NE, USA). The analyzer was set at the PAR of 650 μmol∙m−2∙s−1, 400 µmol∙m−1 CO2 and block temperature of 25 °C. The fully-expanded leaves from each biological replicate were placed in the leaf chamber.

      Leaf EL was used to indicate the cellular membrane stability following treatment and was calculated as (Cinitial/Cmax) × 100%[28]. Fresh leaves (0.2 g) were collected in each test tube containing 35 mL of deionized water. Leaf samples were placed on a shaker for 24 h to measure Cinitial by using a conductivity meter (Orion Star A212, Thermo Scientific Inc., Waltham, MA, USA). Samples were killed by being autoclaved at 121 °C for 20 min and shaken for another 24 h. The final conductance reading (Cmax) of killed leaves were then recorded.

      MDA was measured according to the method documented by Xu et al.[29] with some modifications. Leaves (0.35 g) were ground and 3 mL of 50 mM cold PBS (pH = 7.8) and 1 mL 1 mM EDTA-Na2 were added to a test tube. Samples were centrifuged at 15000 g for 30 min at 4 °C, and then the supernatant was collected for MDA determination. Two mL of supernatant was mixed with 1 mL of reaction solution (20% trichloroacetic acid with 0.5% thiobarbituric acid) and then incubated at 95 °C for 30 min. The mixture was quickly cooled on ice and centrifuged at 10,000 g for 10 min at 4 °C. The supernatant was taken to determine absorbance at 450 nm, 532 nm and 600 nm by using a spectrophotometer (Ultrospec 2100 pro, Biochrom Ltd., Cambridge, UK).

    • Statistical analysis was performed using IBM SPSS Statistics (SPSS 21.0; SPSS Inc., Chicago, IL, USA). ANOVA analysis was used to determine whether exogenous chitosan had a significant impact on creeping bentgrass. Duncan multiple comparison was used to analyze the significant difference of turf quality, ratio of yellow leaves, growth rate, root length, root-shoot ratio, biomass and physiological indexes (Pn, Fv/Fm, EL and MDA) among different treatments at the confidence level of 0.05. The values of all column charts were expressed as mean ± standard error (SE) at the level of 0.05.

    • Turf quality was gradually decreased after high temperature treatment during the experimental period (Fig. 1a). Plants maintained the higher turf quality with foliar application of chitosan from 14 to 49 d compared with the untreated control under heat stress, and difference between different concentrations of chitosan (50, 100 and 500 mg·L−1) was not significant (Fig. 1a). At 49 d of heat stress, turf quality treated with exogenous chitosan was higher than the control by 17.5%, 15.8% and 11.9% under concentrations of 50, 100 and 500 mg·L−1, respectively. The shoot phenotypes with exogenous chitosan under heat stress were consistent with measurement that plants with chitosan application showed obviously superior performance (Fig. 1b).

      Figure 1. 

      Effects of different chitosan concentrations on (a) turf quality and (b) shoot phenotypes in creeping bentgrass under heat stress. Vertical bars indicate significant difference based on LSD values at 0.05 level for the comparison among treatments.

      High temperature led to a rapid increase in the ratio of yellow leaves during the experimental period (Fig. 2a). The lower degree of ratio of yellow leaves in creeping bentgrass applied with chitosan than untreated control was manifested at 7 and 21 d. There was no significant effect on ratio of yellow leaves observed by exogenous application of different chitosan concentrations at 28 d through 49 d of heat stress (Fig. 2a). Foliar application of chitosan did not result in a significant effect on growth rate (Fig. 2b).

      Figure 2. 

      Effects of different chitosan concentrations on (a) ratio of yellow leaves and (b) growth rate in creeping bentgrass under heat stress. Vertical bars indicate significant difference based on LSD values at 0.05 level for the comparison among treatments. Different lowercase letters represent significant difference between different treatments during the experimental period (P < 0.05). Error bars represent standard error (SE).

    • At the end of the experiment, root length was longer than the control by 31.5% and 47.7%, respectively, in foliar chitosan-treated creeping bentgrasses with both 50 and 100 mg·L−1, but the difference between 500 mg·L−1 chitosan and control was not significant under heat stress (Fig. 3a). The effects of treatment with application of chitosan on root growth could be well reflected by root phenotype (Fig. 3b).

      Figure 3. 

      Effects of different chitosan concentrations on (a) root length and (b) root phenotypes in creeping bentgrass under heat stress. Different lowercase letters represent significant difference between different treatments during the experimental period (P < 0.05). Error bars represent standard error (SE).

      The positive effects in both shoot biomass and root biomass were observed due to the foliar application of chitosan under heat conditions (Fig. 4). Shoot biomass significantly increased with chitosan treatments of 50, 100 and 500 mg·L−1 by 59.9%, 95.0% and 93.3% higher than the control, respectively (Fig. 4a). The chitosan-induced increases with concentrations of 100 and 500 mg·L−1 in shoot biomass were greater than that of 50 mg·L−1 chitosan. The effect of exogenous chitosan on root biomass was similar to that of shoot biomass. Plants treated with the chitosan application of 50, 100 and 500 mg·L−1 had significantly larger root biomass by 4.2-fold, 9.0-fold and 10.6-fold than the control at the end of the experiment, respectively. Chitosan-treated plants with 50, 100 and 500 mg·L−1 resulted in a significant increase in root-shoot ratio by 3.0-fold, 4.0-fold and 5.1-fold compared with the control, respectively (Fig. 4b). Furthermore, root-shoot ratio applied by 500 mg·L−1 chitosan was significantly higher by 53.2% than 50 mg·L−1 chitosan.

      Figure 4. 

      Effects of different chitosan concentrations on (a) biomass and (b) root-shoot ratio in creeping bentgrass under heat stress. Different lowercase letters represent significant difference between different treatments during the experimental period (P < 0.05). Error bars represent standard error (SE).

    • No significant difference was detected in Pn and Fv/Fm in both chitosan-treated and untreated plants under non-stressed condition (Fig. 5a, b). High temperature resulted in a rapid decline in Pn and Fv/Fm during the experimental period. However, Pn in chitosan-treated plants was significantly higher than that without chitosan application by 74.8% at 21 d of heat stress (Fig. 5a). At 42 d of treatment, plants without foliar application of chitosan under heat stress had a negative Pn value which was significantly lower than that of chitosan-treated plants. For Fv/Fm, plants with exogenous chitosan had significantly lower decline than chitosan-untreated plants at 21 and 42 d of heat stress (Fig. 5b).

      Figure 5. 

      Effects of chitosan on (a) photosynthetic rate and (b) photochemical efficiency in creeping bentgrass. Control + H2O, plants were sprayed with 10 mL deionized water under 25/20 °C (day/night); Control + CTS, plants were treated foliar application with 10 mL 100 mg·L−1 chitosan under non-stressed condition (25/20 °C, day/night); H + H2O, plants were sprayed with 10 mL deionized water without chitosan under heat stress (38/28 °C, day/night); H + CTS, plants were applied with exogenous chitosan (10 mL, 100 mg·L−1) under heat stress (38/28 °C, day/night). Different lowercase letters represent significant difference between different treatments during the experimental period (P < 0.05). Error bars represent standard error (SE).

    • EL and MDA content were maintained at a normal level under non-stressed conditions and no significant difference was found among different treatments (Fig. 6a, b). High temperature stress significantly increased EL and MDA content compared to control with or without chitosan application during the treatment time. Under heat stress, plants treated with chitosan application had a significantly lower EL by 40% and 41% than chitosan-untreated plants at 21 and 42 d of treatment, respectively (Fig. 6a). In addition, there was no significant difference between chitosan-treated and untreated plants at 21 d of heat stress, but MDA content in plants treated with chitosan declined by 25% compared with plants without chitosan application at 42 d (Fig. 6b).

      Figure 6. 

      Effects of chitosan on (a) electrolyte leakage and (b) malondialdehyde content in creeping bentgrass. Control + H2O, plants were sprayed with 10 mL deionized water under 25/20 °C (day/night); Control + CTS, plants were treated foliar application with 10 mL 100 mg·L−1 chitosan under non-stressed condition (25/20 °C, day/night); H + H2O, plants were sprayed with 10 mL deionized water without chitosan under heat stress (38/28 °C, day/night); H + CTS, plants were applied with exogenous chitosan (10 mL, 100 mg·L−1) under heat stress (38/28 °C, day/night). Different lowercase letters represent significant difference between different treatments during the experimental period (P < 0.05). Error bars represent standard error (SE).

    • High temperature, as one of the major abiotic stresses restricting the growth of cool-season turfgrass, is an important and difficult problem for turfgrass management, especially during summer[3]. Heat stress induced the limitation of physiological and metabolism in various turfgrass species[3032]. The heat-induced damage also occurred in the morphology and growth of plants, especially when turfgrass species suffered continuous severe heat stress[33]. It was previously found that heat-tolerant species usually had better growth performance and physiological characteristics than heat-sensitive plants in creeping bentgrass[9, 34]. In the present study, exogenous chitosan with 100 mg·L−1 significantly enhanced thermotolerance in association with superior plant growth indexes (Figs 1, 3 & 4), photosynthesis (Fig. 5) and cell membrane stability (Fig. 6) compared with the untreated plants under heat stress in creeping bentgrass as discussed below.

      Shoot growth and development were directly associated with turf quality in turfgrass. Studies have shown that high temperature stress would reduce turf quality and increase the ratio of yellow leaves in creeping bentgrass[35]. Turf quality and canopy height also significantly decreased due to heat stress in perennial ryegrass (Lolium perenne) [36]. Chitosan serves as not only a plant growth regulator to promote growth and development of various plants, but also an abiotic stress tolerance inducer[3739]. In our study, foliar application of chitosan improved turf quality and shoot biomass in response to heat stress during the experimental period (Figs 1 & 4a). Similar result was also found by Younas et al.[40] that growth and yield characteristics of maize including shoot dry weight, cob weight and grain yield were improved by applying chitosan under water deficit conditions. Furthermore, chitosan treatment improved growth and quality in soybean sprouts (Glycine max)[41], increased shoot height and number of nodes in potato (Solanum tuberosum)[42]. Chitosan-induced increase in turf quality and biomass in this study indicated that chitosan could enhance heat tolerance and promote plant growth in creeping bentgrass under high temperature conditions.

      Heat stress also leads to injury of belowground root as well as the aboveground shoot. Roots are the main organs for acquiring water and mineral nutrients from the soil in plants[43]. However, root system are more sensitive to heat stress in comparison to shoots[31]. In general, root growth was positively correlated with heat tolerance as reported in various cool-season turfgrass species, such as in tall fescue (L. arundinaceum), perennial ryegrass and creeping bentgrass[10,44,45]. Hence, root growth played a crucial role in enhancing thermotolerance in turfgrass species in response to heat stress. In our study, foliar application of chitosan led to the significant promotion of root length, root-shoot ratio and root biomass compared with the untreated control (Figs 3 & 4). The positive effects of chitosan in thermotolerance through facilitating plant roots have been widely documented in several previous studies. For example, chitosan alleviated adverse effects of salt stress through inhibiting the decline in root length in maize seedlings[24]. Chitosan also mitigated the suppression of root growth in maize under cadmium stress as reflected by higher root length, root surface and root volume[46]. Those results suggested that chitosan played a positive role during root growth under high temperature stress.

      Various physiological activities in plants were limited by high temperature of which photosynthesis as one of physiological processes is sensitive to external temperature. Heat-induced negative effects were observed when creeping bentgrass was subjected to heat stress, as reflected by reduction in chlorophyll content, Fv/Fm and Pn as well as inhibition in rubisco activity and rubisco activation state involving in photosynthesis[1, 47]. In this study, a significant decrease in both Fv/Fm and Pn was detected in plants due to imposition of heat stress, but exogenous chitosan effectively alleviated the decline in Fv/Fm and Pn (Fig. 5). It has also been found that chitosan pretreatment significantly mitigated the adverse effect on Fv/Fm in creeping bentgrass under salt stress[25]. Application of irradiated chitosan significantly increased chlorophyll content and Pn as well as enhanced drought tolerance of sugarcane (Saccharum spp.)[48]. Similarly, the result in this present study demonstrated that chitosan alleviated severe damage in photosynthetic system caused by high temperature. Furthermore, high temperature also led to electrolyte leakage and membrane lipid peroxidation resulting from cell membrane damage[49]. In our study, both EL and MDA content in plants without chitosan treatment significantly increased, but chitosan application significantly inhibited the increase in EL and MDA content under high temperature stress (Fig. 6). This result was also reported by other studies in several plant species, such as in potato[42], thyme (Thymus daenensis)[17] and edible rape (Brassica rapa)[50] indicating that exogenous chitosan contributed to the promotive effects on cell membrane stability and integrity and improved heat tolerance in creeping bentgrass.

      In summary, foliar application of chitosan significantly enhanced heat tolerance as reflected through promoting both shoot and root growth in creeping bentgrass as shown by higher turf quality, root length, root-shoot ratio and biomass. Furthermore, chitosan application also could inhibit the decline in photosynthesis and maintain cell membrane stability. The mechanisms involved in chitosan-induced thermotolerance at the molecular level under heat conditions need to be further investigated in our next studies. This study will provide new insight for turf managers to enhance heat tolerance of perennial turfgrass species, especially during the summer season in cool-season grasses.

      • This research was supported by the Chongqing Key Laboratory of Germplasm Innovation and Utilization of Native Plants (XTZW2021-KF04) and Innovation and Promotion of Forestry Science and Technology of Jiangsu Province (LYKJ[2021]29).

      • The authors declare that they have no conflict of interest.

      • Copyright: © 2022 by the author(s). Published by Maximum Academic Press, Fayetteville, GA. This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
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    Cite this article
    Li Q, Li R, He F, Yang Z, Yu J. 2022. Growth and physiological effects of chitosan on heat tolerance in creeping bentgrass (Agrostis stolonifera). Grass Research 2:6 doi: 10.48130/GR-2022-0006
    Li Q, Li R, He F, Yang Z, Yu J. 2022. Growth and physiological effects of chitosan on heat tolerance in creeping bentgrass (Agrostis stolonifera). Grass Research 2:6 doi: 10.48130/GR-2022-0006

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