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This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Strategic engineering for detecting antimicrobial compounds from Taxus wallichiana Zucc. (Himalayan yew)

  • 1.

    Central Ayurveda Research Institute, Bidhannagar- 700091, Kolkata, West Bengal, India

  • 2.

    G.B. Pant National Institute of Himalayan Environment-Himachal Pradesh Regional Centre, Mohal-Kullu, Himachal Pradesh-175126, India

  • 3.

    Department of Biotechnology, Graphic Era (Deemed to be University), Clement Town, Dehradun-248002, Uttarakhand, India

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  • Taxus wallichiana Zucc. (Himalayan yew) has been well-documented for therapeutically significant active ingredients. Its bark contains pharmaceutically important compounds i.e., taxol and its derivatives which are well known for their anticancer potential. However, T. wallichiana has received limited attention for its equally significant antimicrobial properties. Keeping this background in view, T. wallichiana was selected for the detailed investigation of, antimicrobial activities and isolation and characterization of secondary metabolites responsible for antimicrobial activity in different plant parts i.e., needle, bark, and stem extracts. In plate-based bioassays, plants exhibited antimicrobial action against the three main categories of microorganisms (fungi, bacteria, and actinobacteria). Based on the preliminary antimicrobial study, methanol and ethyl acetate extracts, were selected for further experiments. The bioautographic technique was used for identification, and the mobile phase was optimized with the help of a selectivity triangle. After continuous column and thin-layer chromatography, fractions were identified as having good antifungal, antibacterial, and antiactinobacterial activity. These fractions were selected for further characterization using techniques like GC-MS/ LC-MS, and FTIR. These analyses support the identification of several fatty acids, including arachidic acid, behenic acid, palmitic acid, and stearic acid; vitamins (nicotinamide); alkaloids (cinchonine, timolol); amino benzamides (procainamide); carbocyclic sugars (myoinositol); and alkane hydrocarbons (hexadecane), which have antimicrobial activity in T. wallichiana needles. The information gathered from this study will help modern medicine make new drug discoveries that combine different active ingredients from medicinal plants to treat a wide range of ailments.

  • Cite this article

    Adhikari P, Agnihotri V, Pandey A. 2024. Strategic engineering for detecting antimicrobial compounds from Taxus wallichiana Zucc. (Himalayan yew). Medicinal Plant Biology doi: 10.48130/mpb-0024-0020
    Adhikari P, Agnihotri V, Pandey A. 2024. Strategic engineering for detecting antimicrobial compounds from Taxus wallichiana Zucc. (Himalayan yew). Medicinal Plant Biology doi: 10.48130/mpb-0024-0020
    shu

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Important Notice

This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Strategic engineering for detecting antimicrobial compounds from Taxus wallichiana Zucc. (Himalayan yew)

  • 1.

    Central Ayurveda Research Institute, Bidhannagar- 700091, Kolkata, West Bengal, India

  • 2.

    G.B. Pant National Institute of Himalayan Environment-Himachal Pradesh Regional Centre, Mohal-Kullu, Himachal Pradesh-175126, India

  • 3.

    Department of Biotechnology, Graphic Era (Deemed to be University), Clement Town, Dehradun-248002, Uttarakhand, India

  • Received: 30 April 2024
  • Revised: 25 July 2024
  • Accepted: 26 July 2024
    • First-online: 14 August 2024
Medicinal Plant Biology  Article in press  ()  |  Cite this article

Abstract: Taxus wallichiana Zucc. (Himalayan yew) has been well-documented for therapeutically significant active ingredients. Its bark contains pharmaceutically important compounds i.e., taxol and its derivatives which are well known for their anticancer potential. However, T. wallichiana has received limited attention for its equally significant antimicrobial properties. Keeping this background in view, T. wallichiana was selected for the detailed investigation of, antimicrobial activities and isolation and characterization of secondary metabolites responsible for antimicrobial activity in different plant parts i.e., needle, bark, and stem extracts. In plate-based bioassays, plants exhibited antimicrobial action against the three main categories of microorganisms (fungi, bacteria, and actinobacteria). Based on the preliminary antimicrobial study, methanol and ethyl acetate extracts, were selected for further experiments. The bioautographic technique was used for identification, and the mobile phase was optimized with the help of a selectivity triangle. After continuous column and thin-layer chromatography, fractions were identified as having good antifungal, antibacterial, and antiactinobacterial activity. These fractions were selected for further characterization using techniques like GC-MS/ LC-MS, and FTIR. These analyses support the identification of several fatty acids, including arachidic acid, behenic acid, palmitic acid, and stearic acid; vitamins (nicotinamide); alkaloids (cinchonine, timolol); amino benzamides (procainamide); carbocyclic sugars (myoinositol); and alkane hydrocarbons (hexadecane), which have antimicrobial activity in T. wallichiana needles. The information gathered from this study will help modern medicine make new drug discoveries that combine different active ingredients from medicinal plants to treat a wide range of ailments.

    Conflict of interest

    • The authors declare that they have no conflict of interest.

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    • Copyright: © 2024 by the author(s). Published by Maximum Academic Press, Fayetteville, GA. This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
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    Cite this article
    Adhikari P, Agnihotri V, Pandey A. 2024. Strategic engineering for detecting antimicrobial compounds from Taxus wallichiana Zucc. (Himalayan yew). Medicinal Plant Biology doi: 10.48130/mpb-0024-0020
    Adhikari P, Agnihotri V, Pandey A. 2024. Strategic engineering for detecting antimicrobial compounds from Taxus wallichiana Zucc. (Himalayan yew). Medicinal Plant Biology doi: 10.48130/mpb-0024-0020
    shu

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