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This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Analysis of monoterpene biosynthesis and functional TPSs of Perilla frutescens based on transcriptome and metabolome

  • 1.

    Institute of Medical Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

  • 2.

    Featured Laboratory for Biosynthesis and Target Discovery of Active Components of Traditional Chinese Medicine, School of Integrated Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, China

  • 3.

    The Institute of Medicinal Plant Development, the Chinese Academy of Medical Sciences, The Institute of Medicinal Plant Development (IMPLAD), Beijing 100193, China

  • 4.

    Guizhou Institute of Subtropical Crop, Guizhou Academy of Agricultural Sciences, Guiyang 550025, China

  • # Authors contributed equally: Shi-Mei Yang, Hong-Ye Chu

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  • Perilla is a characteristic medicinal and edible plant. According to the different main monoterpenes in Perilla leaves, Perilla can be divided into many chemical types. In this study, four Perilla cultivars of different chemical types, included perillaldehyde (PA), perillaketone (PK), perillene (PL) and piperitenone (PT) were selected for metabolome and transcriptome analysis. Totally, 35 terpene compounds were identified and main monoterpenes were consistent with corresponding chemical types, respectively. Combined with transcriptome, a total of 5,920 differentially expressed genes were obtained among them. Totally, 69 genes refered to MVA and MEP pathways and 109 PfTPSs encoded genes were identified, which possessed partial differentially expressed in four chemical types. The core PfTPSs in co-expression analysis were functional characterization. Three PfTPSs were identified as linalool synthase and one PfTPSs were identified as geranyl synthase. This research analyzed the monoterpene biosynthesis and functional characterization of TPSs in Perilla, which can give foundation for in-depth research of Perilla chemotype metabolic mechanism.

  • Cite this article

    Yang SM, Chu HY, Wang YX, Guo BL, An TY, et al. 2024. Analysis of monoterpene biosynthesis and functional TPSs of Perilla frutescens based on transcriptome and metabolome. Medicinal Plant Biology doi: 10.48130/mpb-0024-0017
    Yang SM, Chu HY, Wang YX, Guo BL, An TY, et al. 2024. Analysis of monoterpene biosynthesis and functional TPSs of Perilla frutescens based on transcriptome and metabolome. Medicinal Plant Biology doi: 10.48130/mpb-0024-0017
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Important Notice

This is accepted manuscript by the journal but prior to copy-editing or proofing. It can be cited using the author(s), article title, journal title, year of online publication, and DOI. It will be replaced by the final typeset version, which may therefore contain changes. The DOI will be remain the same.

ARTICLE   Open Access    

Analysis of monoterpene biosynthesis and functional TPSs of Perilla frutescens based on transcriptome and metabolome

  • 1.

    Institute of Medical Plant Physiology and Ecology, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China

  • 2.

    Featured Laboratory for Biosynthesis and Target Discovery of Active Components of Traditional Chinese Medicine, School of Integrated Traditional Chinese and Western Medicine, Binzhou Medical University, Yantai 264003, China

  • 3.

    The Institute of Medicinal Plant Development, the Chinese Academy of Medical Sciences, The Institute of Medicinal Plant Development (IMPLAD), Beijing 100193, China

  • 4.

    Guizhou Institute of Subtropical Crop, Guizhou Academy of Agricultural Sciences, Guiyang 550025, China

  • Received: 02 February 2024
  • Accepted: 24 June 2024
    • First-online: 09 July 2024
Medicinal Plant Biology  Article in press  (2024)  |  Cite this article

Abstract: Perilla is a characteristic medicinal and edible plant. According to the different main monoterpenes in Perilla leaves, Perilla can be divided into many chemical types. In this study, four Perilla cultivars of different chemical types, included perillaldehyde (PA), perillaketone (PK), perillene (PL) and piperitenone (PT) were selected for metabolome and transcriptome analysis. Totally, 35 terpene compounds were identified and main monoterpenes were consistent with corresponding chemical types, respectively. Combined with transcriptome, a total of 5,920 differentially expressed genes were obtained among them. Totally, 69 genes refered to MVA and MEP pathways and 109 PfTPSs encoded genes were identified, which possessed partial differentially expressed in four chemical types. The core PfTPSs in co-expression analysis were functional characterization. Three PfTPSs were identified as linalool synthase and one PfTPSs were identified as geranyl synthase. This research analyzed the monoterpene biosynthesis and functional characterization of TPSs in Perilla, which can give foundation for in-depth research of Perilla chemotype metabolic mechanism.

    Acknowledgments

    • This work was funded by the National Natural Science Foundation of China Grant (U22A20446) and the National Natural Science Foundation for Regional Fund (31860391).

    Conflict of interest

    • The authors declare that they have no conflict of interest.

    • # Authors contributed equally: Shi-Mei Yang, Hong-Ye Chu

    Rights and permissions
    • Copyright: © 2024 by the author(s). Published by Maximum Academic Press, Fayetteville, GA. This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
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    Yang SM, Chu HY, Wang YX, Guo BL, An TY, et al. 2024. Analysis of monoterpene biosynthesis and functional TPSs of Perilla frutescens based on transcriptome and metabolome. Medicinal Plant Biology doi: 10.48130/mpb-0024-0017
    Yang SM, Chu HY, Wang YX, Guo BL, An TY, et al. 2024. Analysis of monoterpene biosynthesis and functional TPSs of Perilla frutescens based on transcriptome and metabolome. Medicinal Plant Biology doi: 10.48130/mpb-0024-0017
    shu

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