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2023 Volume 3
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REVIEW   Open Access    

Role of proline in regulating turfgrass tolerance to abiotic stress

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  • Turfgrasses used on golf courses, sports fields and in urban landscapes have significant ecological and economic value. Various abiotic stresses are crucial in limiting the growth and development of turfgrass. As an important amino acid metabolite, proline can act as a signal to trigger plant responses to stress. Proline accumulation is not only a stress signal, but also alleviates plant damage by maintaining photosynthesis and the activities of antioxidant enzymes and the levels of non-enzymatic antioxidant compounds, thus reducing reactive oxygen species content, and regulating osmosis. A better understanding of mechanisms of proline response to abiotic stress in turfgrass is vital for the development of stress-tolerant germplasm. This review summarizes research progress into the role of proline in regulating growth and physiological and molecular adaptations to abiotic stress, with emphasis on drought, salt and temperature tolerance.
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  • [1]

    Huang B. 2021. Grass research for a productive, healthy and sustainable society. Grass Research 1:1

    doi: 10.48130/gr-2021-0001

    CrossRef   Google Scholar

    [2]

    Qian Y, Follett RF. 2002. Assessing Soil Carbon Sequestration in Turfgrass Systems Using Long-Term Soil Testing Data. Agronomy Journal 94:930−35

    doi: 10.2134/agronj2002.9300

    CrossRef   Google Scholar

    [3]

    Chen Y, Pettersen T, Kvalbein A, Aamlid TS. 2018. Playing quality, growth rate, thatch accumulation and tolerance to moss and annual bluegrass invasion as influenced by irrigation strategies on red fescue putting greens. Journal of Agronomy and Crop Science 204:185−95

    doi: 10.1111/jac.12246

    CrossRef   Google Scholar

    [4]

    Lai J, Han L. 2022. Progress and challenges in China turfgrass abiotic stress resistance research. Frontiers in Plant Science 13:922175

    doi: 10.3389/fpls.2022.922175

    CrossRef   Google Scholar

    [5]

    Alvarez ME, Savouré A, Szabados L. 2022. Proline metabolism as regulatory hub. Trends in Plant Science 27:39−55

    doi: 10.1016/j.tplants.2021.07.009

    CrossRef   Google Scholar

    [6]

    Cao L, Han L, Zhang H, Xin H, Imtiaz M, et al. 2015. Isolation and characterization of pyrroline-5-carboxylate synthetase gene from perennial ryegrass (Lolium perenne L. ). Acta Physiologiae Plantarum 37:62

    doi: 10.1007/s11738-015-1808-9

    CrossRef   Google Scholar

    [7]

    Szabados L, Savouré A. 2010. Proline: A multifunctional amino acid. Trends in Plant Science 15:89−97

    doi: 10.1016/j.tplants.2009.11.009

    CrossRef   Google Scholar

    [8]

    Huang B, DaCosta M, Jiang Y. 2014. Research advances in mechanisms of turfgrass tolerance to abiotic stresses: from physiology to molecular biology. Critical Reviews in Plant Sciences 33:141−89

    doi: 10.1080/07352689.2014.870411

    CrossRef   Google Scholar

    [9]

    Bocian A, Zwierzykowski Z, Rapacz M, Koczyk G, Ciesiołka D, Kosmala A. 2015. Metabolite profiling during cold acclimation of Lolium perenne genotypes distinct in the level of frost tolerance. Journal of Applied Genetics 56:439−49

    doi: 10.1007/s13353-015-0293-6

    CrossRef   Google Scholar

    [10]

    Chan Z, Shi H. 2015. Improved abiotic stress tolerance of bermudagrass by exogenous small molecules. Plant Signaling & Behavior 10:e991577

    doi: 10.4161/15592324.2014.991577

    CrossRef   Google Scholar

    [11]

    Zuo ZF, Kang HG, Park MY, Jeong H, Sun HJ, et al. 2019. Zoysia japonica MYC type transcription factor ZjICE1 regulates cold tolerance in transgenic Arabidopsis. Plant Science 289:110254

    doi: 10.1016/j.plantsci.2019.110254

    CrossRef   Google Scholar

    [12]

    Katuwal KB, Xiao B, Jespersen D. 2020. Physiological responses and tolerance mechanisms of seashore paspalum and centipedegrass exposed to osmotic and iso-osmotic salt stresses. Journal of Plant Physiology 248:153154

    doi: 10.1016/j.jplph.2020.153154

    CrossRef   Google Scholar

    [13]

    Fichman Y, Gerdes SY, Kovács H, Szabados L, Zilberstein A, et al. 2015. Evolution of proline biosynthesis: enzymology, bioinformatics, genetics, and transcriptional regulation. Biological Reviews 90:1065−99

    doi: 10.1111/brv.12146

    CrossRef   Google Scholar

    [14]

    Ashraf M, Foolad MR. 2007. Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany 59:206−16

    doi: 10.1016/j.envexpbot.2005.12.006

    CrossRef   Google Scholar

    [15]

    Matysik J, Alia A, Bhalu B, Mohanty P. 2002. Molecular mechanisms of quenching of reactive oxygen species by proline under stress in plants. Current Science 82:525−32

    Google Scholar

    [16]

    Smirnoff N, Cumbes QJ. 1989. Hydroxyl radical scavenging activity of compatible solutes. Phytochemistry 28:1057−60

    doi: 10.1016/0031-9422(89)80182-7

    CrossRef   Google Scholar

    [17]

    Schat H, Sharma SS, Vooijs R. 1997. Heavy metal-induced accumulation of free proline in a metal-tolerant and a nontolerant ecotype of Silene vulgaris. Physiologia Plantarum 101:477−82

    doi: 10.1111/j.1399-3054.1997.tb01026.x

    CrossRef   Google Scholar

    [18]

    Sharma SS, Dietz KJ. 2006. The significance of amino acids and amino acid-derived molecules in plant responses and adaptation to heavy metal stress. Journal of Experimental Botany 57:711−26

    doi: 10.1093/jxb/erj073

    CrossRef   Google Scholar

    [19]

    Verbruggen N, Villarroel R, Van Montagu M. 1993. Osmoregulation of a pyrroline-5-carboxylate reductase gene in Arabidopsis thaliana. Plant Physiology 103:771−81

    doi: 10.1104/pp.103.3.771

    CrossRef   Google Scholar

    [20]

    Hong Z, Lakkineni K, Zhang Z, Verma DP. 2000. Removal of feedback inhibition of Δ1-pyrroline-5-carboxylate synthetase results in increased proline accumulation and protection of plants from osmotic stress. Plant Physiology 122:1129−36

    doi: 10.1104/pp.122.4.1129

    CrossRef   Google Scholar

    [21]

    Ghosh UK, Islam MN, Siddiqui MN, Cao X, Khan MAR. 2022. Proline, a multifaceted signalling molecule in plant responses to abiotic stress: understanding the physiological mechanisms. Plant Biology 24:227−39

    doi: 10.1111/plb.13363

    CrossRef   Google Scholar

    [22]

    Aalipour H, Nikbakht A, Ghasemi M, Amiri R. 2020. Morpho-physiological and biochemical responses of two turfgrass species to arbuscular mycorrhizal fungi and humic acid under water stress condition. Journal of Soil Science and Plant Nutrition 20:566−76

    doi: 10.1007/s42729-019-00146-4

    CrossRef   Google Scholar

    [23]

    Manuchehri R, Salehi H. 2014. Physiological and biochemical changes of common bermudagrass (Cynodon dactylon [L.] Pers.) under combined salinity and deficit irrigation stresses. South African Journal of Botany 92:83−88

    doi: 10.1016/j.sajb.2014.02.006

    CrossRef   Google Scholar

    [24]

    Katuwal KB, Xiao B, Jespersen D. 2020. Root physiological and biochemical responses of seashore paspalum and centipedegrass exposed to iso-osmotic salt and drought stresses. Crop Science 60:1077−89

    doi: 10.1002/csc2.20029

    CrossRef   Google Scholar

    [25]

    Huang S, Jiang S, Liang J, Chen M, Shi Y. 2019. Current knowledge of bermudagrass responses to abiotic stresses. Breeding Science 69:215−26

    doi: 10.1270/jsbbs.18164

    CrossRef   Google Scholar

    [26]

    Zhang L, Zhong T, Xu L, Han L, Zhang X. 2015. Water deficit irrigation impacts on antioxidant metabolism associated with freezing tolerance in creeping bentgrass. Journal of the American Society for Horticultural Science 140:323−32

    doi: 10.21273/JASHS.140.4.323

    CrossRef   Google Scholar

    [27]

    Khoshkholghsima NA, Rohollahi I. 2015. Evaluating biochemical response of some selected perennial grasses under drought stress in Iran. Horticulture, Environment, and Biotechnology 56:383−90

    doi: 10.1007/s13580-015-0010-8

    CrossRef   Google Scholar

    [28]

    Man D, Bao Y, Han L, Zhang X. 2011. Drought tolerance associated with proline and hormone metabolism in two tall fescue cultivars. HortScience horts 46:1027−32

    doi: 10.21273/HORTSCI.46.7.1027

    CrossRef   Google Scholar

    [29]

    Perlikowski D, Augustyniak A, Masajada K, Skirycz A, Soja AM, et al. 2019. Structural and metabolic alterations in root systems under limited water conditions in forage grasses of Lolium-Festuca complex. Plant Science 283:211−23

    doi: 10.1016/j.plantsci.2019.02.001

    CrossRef   Google Scholar

    [30]

    Sarmast MK, Salehi H, Niazi A. 2015. Biochemical differences underlie varying drought tolerance in four Festuca arundinacea Schreb. genotypes subjected to short water scarcity. Acta Physiologiae Plantarum 37:192

    doi: 10.1007/s11738-015-1942-4

    CrossRef   Google Scholar

    [31]

    Chapman C, Rossi S, Yuan B, Huang B. 2022. Differential regulation of amino acids and nitrogen for drought tolerance and poststress recovery in creeping bentgrass. Journal of the American Society for Horticultural Science 147:208−15

    doi: 10.21273/JASHS05215-22

    CrossRef   Google Scholar

    [32]

    Tan M, Hassan MJ, Peng Y, Feng G, Huang L, et al. 2022. Polyamines metabolism interacts with γ-aminobutyric acid, proline and nitrogen metabolisms to affect drought tolerance of creeping bentgrass. International Journal of Molecular Sciences 23:2779

    doi: 10.3390/ijms23052779

    CrossRef   Google Scholar

    [33]

    He A, Niu S, Yang D, Ren W, Zhao L, et al. 2021. Two PGPR strains from the rhizosphere of Haloxylon ammodendron promoted growth and enhanced drought tolerance of ryegrass. Plant Physiology and Biochemistry 161:74−85

    doi: 10.1016/j.plaphy.2021.02.003

    CrossRef   Google Scholar

    [34]

    Shi H, Ye T, Chan Z. 2013. Exogenous application of hydrogen sulfide donor sodium hydrosulfide enhanced multiple abiotic stress tolerance in bermudagrass (Cynodon dactylon (L). Pers.). Plant Physiology and Biochemistry 71:226−34

    doi: 10.1016/j.plaphy.2013.07.021

    CrossRef   Google Scholar

    [35]

    Hatamzadeh A, Molaahmad Nalousi A, Ghasemnezhad M, Biglouei MH. 2015. The potential of nitric oxide for reducing oxidative damage induced by drought stress in two turfgrass species, creeping bentgrass and tall fescue. Grass and Forage Science 70:538−48

    doi: 10.1111/gfs.12135

    CrossRef   Google Scholar

    [36]

    Mahdavi S, Kafi M, Fallahi E, Shokrpour M, Tabrizi L. 2017. Drought and biostimulant impacts on mineral nutrients, ambient and reflected light-based chlorophyll index, and performance of perennial ryegrass. Journal of Plant Nutrition 40:2248−58

    doi: 10.1080/01904167.2016.1237650

    CrossRef   Google Scholar

    [37]

    Sheikh Mohammadi MH, Etemadi N, Arab MM, Aalifar M, Arab M, et al. 2017. Molecular and physiological responses of Iranian perennial ryegrass as affected by Trinexapac ethyl, Paclobutrazol and Abscisic acid under drought stress. Plant Physiology and Biochemistry 111:129−43

    doi: 10.1016/j.plaphy.2016.11.014

    CrossRef   Google Scholar

    [38]

    Zhang N, Han L, Xu L, Zhang X. 2018. Ethephon seed treatment impacts on drought tolerance of Kentucky bluegrass seedlings. HortTechnology 28:319−26

    doi: 10.21273/HORTTECH03976-18

    CrossRef   Google Scholar

    [39]

    Chen Z, Wang Z, Yang Y, Li M, Xu B. 2018. Abscisic acid and brassinolide combined application synergistically enhances drought tolerance and photosynthesis of tall fescue under water stress. Scientia Horticulturae 228:1−9

    doi: 10.1016/j.scienta.2017.10.004

    CrossRef   Google Scholar

    [40]

    Li Z, Fu J, Shi D, Peng Y. 2020. Myo-inositol enhances drought tolerance in creeping bentgrass through alteration of osmotic adjustment, photosynthesis, and antioxidant defense. Crop Science 60:2149−58

    doi: 10.1002/csc2.20186

    CrossRef   Google Scholar

    [41]

    Zhang J, Gao Y, Xu L, Han L. 2021. Transcriptome analysis of Kentucky bluegrass subject to drought and ethephon treatment. PLoS One 16:e0261472

    doi: 10.1371/journal.pone.0261472

    CrossRef   Google Scholar

    [42]

    Saud S, Fahad S, Cui G, Chen Y, Anwar S. 2020. Determining nitrogen isotopes discrimination under drought stress on enzymatic activities, nitrogen isotope abundance and water contents of Kentucky bluegrass. Scientific Reports 10:6415

    doi: 10.1038/s41598-020-63548-w

    CrossRef   Google Scholar

    [43]

    Chen ZL, Li XM, Zhang LH. 2014. Effect of salicylic acid pretreatment on drought stress responses of zoysiagrass (Zoysia japonica). Russian Journal of Plant Physiology 61:619−25

    doi: 10.1134/S1021443714050057

    CrossRef   Google Scholar

    [44]

    Ma Y, Shukla V, Merewitz EB. 2017. Transcriptome analysis of creeping bentgrass exposed to drought stress and polyamine treatment. PLoS One 12:e0175848

    doi: 10.1371/journal.pone.0175848

    CrossRef   Google Scholar

    [45]

    Liu N, Shen Y, Huang B. 2015. Osmoregulants involved in osmotic adjustment for differential drought tolerance in different bentgrass genotypes. Journal of the American Society for Horticultural Science 140:605−13

    doi: 10.21273/JASHS.140.6.605

    CrossRef   Google Scholar

    [46]

    Li J, Ma J, Guo H, Zong J, Chen J, et al. 2018. Growth and physiological responses of two phenotypically distinct accessions of centipedegrass (Eremochloa ophiuroides (Munro) Hack.) to salt stress. Plant Physiology and Biochemistry 126:1−10

    doi: 10.1016/j.plaphy.2018.02.018

    CrossRef   Google Scholar

    [47]

    Uddin MK, Juraimi AS. 2013. Salinity tolerance turfgrass: history and prospects. Scientific World Journal 2013:409413

    doi: 10.1155/2013/409413

    CrossRef   Google Scholar

    [48]

    Xu R, Fujiyama H. 2013. Comparison of ionic concentration, organic solute accumulation and osmotic adaptation in Kentucky bluegrass and tall fescue under NaCl stress. Soil Science and Plant Nutrition 59:168−79

    doi: 10.1080/00380768.2012.763215

    CrossRef   Google Scholar

    [49]

    Soliman WS, Sugiyama S, Abbas AM. 2018. Contribution of avoidance and tolerance strategies towards salinity stress resistance in eight C3 turfgrass species. Horticulture, Environment, and Biotechnology 59:29−36

    doi: 10.1007/s13580-018-0004-4

    CrossRef   Google Scholar

    [50]

    Liu T, Zhuang L, Huang B. 2019. Metabolic adjustment and gene expression for root sodium transport and calcium signaling contribute to salt tolerance in Agrostis grass species. Plant and Soil 443:219−32

    doi: 10.1007/s11104-019-04140-8

    CrossRef   Google Scholar

    [51]

    Puyang X, An M, Xu L, Han L, Zhang X. 2016. Protective effect of exogenous spermidine on ion and polyamine metabolism in Kentucky bluegrass under salinity stress. Horticulture, Environment, and Biotechnology 57:11−19

    doi: 10.1007/s13580-016-0113-x

    CrossRef   Google Scholar

    [52]

    Sun S, An M, Han L, Yin S. 2015. Foliar application of 24-epibrassinolide improved salt stress tolerance of perennial ryegrass. HortScience 50:1518−23

    doi: 10.21273/HORTSCI.50.10.1518

    CrossRef   Google Scholar

    [53]

    Wu W, Zhang Q, Ervin EH, Yang Z, Zhang X. 2017. Physiological mechanism of enhancing salt stress tolerance of perennial ryegrass by 24-epibrassinolide. Frontiers in Plant Science 8:1017

    doi: 10.3389/fpls.2017.01017

    CrossRef   Google Scholar

    [54]

    Ahmadi F, Nazari F, Ghaderi N, Teixeira da Silva JA. 2023. Assessment of morpho-physiological and biochemical responses of perennial ryegrass to gamma-aminobutyric acid (GABA) application under salinity stress using multivariate analyses techniques. Journal of Plant Growth Regulation 42:168−82

    doi: 10.1007/s00344-021-10538-5

    CrossRef   Google Scholar

    [55]

    Esmaeili S, Salehi H, Eshghi S. 2015. Silicon ameliorates the adverse effects of salinity on turfgrass growth and development. Journal of Plant Nutrition 38:1885−901

    doi: 10.1080/01904167.2015.1069332

    CrossRef   Google Scholar

    [56]

    Li H, Guo H, Zhang X, Fu J. 2014. Expression profiles of Pr5CS1 and Pr5CS2 genes and proline accumulation under salinity stress in perennial ryegrass (Lolium perenne L.). Plant Breeding 133:243−49

    doi: 10.1111/pbr.12140

    CrossRef   Google Scholar

    [57]

    Huang X, Chao D, Gao J, Zhu M, Shi M, et al. 2009. A previously unknown zinc finger protein, DST, regulates drought and salt tolerance in rice via stomatal aperture control. Genes & Development 23:1805−17

    doi: 10.1101/gad.1812409

    CrossRef   Google Scholar

    [58]

    Cen H, Ye W, Liu Y, Li D, Wang K, et al. 2016. Overexpression of a chimeric gene, OsDST-SRDX, improved salt tolerance of perennial ryegrass. Scientific reports 6:27320

    doi: 10.1038/srep27320

    CrossRef   Google Scholar

    [59]

    Li Z, Zeng W, Cheng B, Xu J, Han L, et al. 2022. Turf quality and physiological responses to summer stress in four creeping bentgrass cultivars in a subtropical zone. Plants 11:665

    doi: 10.3390/plants11050665

    CrossRef   Google Scholar

    [60]

    Rossi S, Chapman C, Huang B. 2020. Suppression of heat-induced leaf senescence by γ-aminobutyric acid, proline, and ammonium nitrate through regulation of chlorophyll degradation in creeping bentgrass. Environmental and Experimental Botany 177:104116

    doi: 10.1016/j.envexpbot.2020.104116

    CrossRef   Google Scholar

    [61]

    Rossi S, Chapman C, Yuan B, Huang B. 2021. Improved heat tolerance in creeping bentgrass by γ-aminobutyric acid, proline, and inorganic nitrogen associated with differential regulation of amino acid metabolism. Plant Growth Regulation 93:231−42

    doi: 10.1007/s10725-020-00681-6

    CrossRef   Google Scholar

    [62]

    Chen Y, Guo Z, Dong L, Fu Z, Zheng Q, et al. 2021. Turf performance and physiological responses of native Poa species to summer stress in northeast China. PeerJ 9:e12252

    doi: 10.7717/peerj.12252

    CrossRef   Google Scholar

    [63]

    Xia F, Han Z, Zhu H, Dong K, Du L. 2020. Comparison of osmoprotectants and antioxidant enzymes of different wild Kentucky bluegrass in Shanxi province under high-temperature stress. European Journal of Horticultural Science 85:284−92

    doi: 10.17660/eJHS.2020/85.4.10

    CrossRef   Google Scholar

    [64]

    Sheikh-Mohamadi MH, Etemadi N, Arab M. 2018. Correlation of heat and cold tolerance in Iranian tall fescue ecotypes with reactive oxygen species scavenging and osmotic adjustment. HortScience 53:1062−8

    doi: 10.21273/HORTSCI13088-18

    CrossRef   Google Scholar

    [65]

    Sun T, Shao K, Huang Y, Lei Y, Tan L, et al. 2020. Natural variation analysis of perennial ryegrass in response to abiotic stress highlights LpHSFC1b as a positive regulator of heat stress. Environmental and Experimental Botany 179:104192

    doi: 10.1016/j.envexpbot.2020.104192

    CrossRef   Google Scholar

    [66]

    Xu Y, Huang B. 2018. Transcriptomic analysis reveals unique molecular factors for lipid hydrolysis, secondary cell-walls and oxidative protection associated with thermotolerance in perennial grass. BMC Genomics 19:70

    doi: 10.1186/s12864-018-4437-z

    CrossRef   Google Scholar

    [67]

    Liu M, Sun T, Liu C, Zhang H, Wang W, et al. 2022. Integrated physiological and transcriptomic analyses of two warm- and cool-season turfgrass species in response to heat stress. Plant Physiology and Biochemistry 170:275−86

    doi: 10.1016/j.plaphy.2021.12.013

    CrossRef   Google Scholar

    [68]

    Zhang H, Wang Y, Wang W, Bao M, Chan Z. 2019. Physiological changes and DREB1s expression profiles of tall fescue in response to freezing stress. Scientia Horticulturae 245:116−24

    doi: 10.1016/j.scienta.2018.09.052

    CrossRef   Google Scholar

    [69]

    Chang Z, Sun B, Li D. 2017. Water withholding contributes to winter hardiness in perennial ryegrass (Lolium perenne L.). European Journal for Horticultural Science 82:31−37

    doi: 10.17660/eJHS.2017/82.1.4

    CrossRef   Google Scholar

    [70]

    Sarkar D, Bhowmik PC, Young-In-Kwon, Shetty K. 2009. Cold acclimation responses of three cool-season turfgrasses and the role of proline-associated pentose phosphate pathway. Journal of the American Society for Horticultural Science 134:210−20

    doi: 10.21273/JASHS.134.2.210

    CrossRef   Google Scholar

    [71]

    Hoffman L, DaCosta M, Bertrand A, Castonguay Y, Ebdon JS. 2014. Comparative assessment of metabolic responses to cold acclimation and deacclimation in annual bluegrass and creeping bentgrass. Environmental and Experimental Botany 106:197−206

    doi: 10.1016/j.envexpbot.2013.12.018

    CrossRef   Google Scholar

    [72]

    Gururani MA, Venkatesh J, Ganesan M, Strasser RJ, Han Y, et al. 2015. In vivo assessment of cold tolerance through chlorophyll-α fluorescence in transgenic zoysiagrass expressing mutant phytochrome A. PLoS One 10:e0127200

    doi: 10.1371/journal.pone.0127200

    CrossRef   Google Scholar

    [73]

    Fan J, Zhang W, Amombo E, Hu L, Kjorven JO, Chen L. 2020. Mechanisms of environmental stress tolerance in turfgrass. Agronomy 10:522

    doi: 10.3390/agronomy10040522

    CrossRef   Google Scholar

    [74]

    Wei S, Du Z, Gao F, Ke X, Li J, et al. 2015. Global transcriptome profiles of 'Meyer' zoysiagrass in response to cold stress. PLoS One 10:e0131153

    doi: 10.1371/journal.pone.0131153

    CrossRef   Google Scholar

    [75]

    Dong W, Ma X, Jiang H, Zhao C, Ma H. 2020. Physiological and transcriptome analysis of Poa pratensis var. anceps cv. Qinghai in response to cold stress. BMC Plant Biology 20:362

    doi: 10.1186/s12870-020-02559-1

    CrossRef   Google Scholar

    [76]

    Long S, Yan F, Yang L, Sun Z, Wei S. 2020. Responses of Manila Grass (Zoysia matrella) to chilling stress: From transcriptomics to physiology. PLoS One 15:e0235972

    doi: 10.1371/journal.pone.0235972

    CrossRef   Google Scholar

    [77]

    Feng W, Li J, Long S, Wei S. 2019. A DREB1 gene from zoysiagrass enhances Arabidopsis tolerance to temperature stresses without growth inhibition. Plant Science 278:20−31

    doi: 10.1016/j.plantsci.2018.10.009

    CrossRef   Google Scholar

    [78]

    Zhuang L, Yuan X, Chen Y, Xu B, Yang Z, et al. 2015. PpCBF3 from cold-tolerant Kentucky bluegrass involved in freezing tolerance associated with up-regulation of cold-related genes in transgenic Arabidopsis thaliana. PLoS One 10:e0132928

    doi: 10.1371/journal.pone.0132928

    CrossRef   Google Scholar

    [79]

    Zuo ZF, Kang HG, Park MY, Jeong H, Sun HJ, et al. 2019. Overexpression of ICE1, a regulator of cold-Induced transcriptome, confers cold tolerance to transgenic Zoysia japonica. Journal of Plant Biology 62:137−46

    doi: 10.1007/s12374-018-0330-1

    CrossRef   Google Scholar

    [80]

    Zhang H, Xu Q, Xu X, Liu HB, Zhi JK, et al. 2017. Transgenic tobacco plants expressing grass AstEXPA1 gene show improved performance to several stresses. Plant Biotechnology Reports 11:331−7

    doi: 10.1007/s11816-017-0454-7

    CrossRef   Google Scholar

    [81]

    Zhang H, Shi Y, Liu X, Wang R, Li J, Xu J. 2018. Transgenic creeping bentgrass plants expressing a Picea wilsonii dehydrin gene (PicW) demonstrate improved freezing tolerance. Molecular Biology Reports 45:1627−35

    doi: 10.1007/s11033-018-4304-7

    CrossRef   Google Scholar

    [82]

    Li X, Wang L, Li Y, Sun L, Cai S, et al. 2014. Comparative analyses of physiological responses of Cynodon dactylon accessions from Southwest China to sulfur dioxide toxicity. The Scientific World Journal 2014:916595

    doi: 10.1155/2014/916595

    CrossRef   Google Scholar

    [83]

    Li X, Cen H, Peng L, Li Y, Sun L, et al. 2015. Tolerance performance of the cool-season turfgrass species Festuca ovina, Lolium perenne, Agrostis tenuis, and Poa trivialis to sulfur dioxide stress. Journal of Plant Interactions 10:75−86

    doi: 10.1080/17429145.2015.1019984

    CrossRef   Google Scholar

    [84]

    Yang WZ, Fu JJ, Yang LY, Zhang X, Zheng YL, et al. 2014. Protective effects of complementary Ca2+ on low-light-induced oxidative damage in tall fescue. Russian Journal of Plant Physiology 61:818−27

    doi: 10.1134/S1021443714060211

    CrossRef   Google Scholar

    [85]

    Jia H, Hou D, O’Connor D, Pan S, Zhu J, et al. 2020. Exogenous phosphorus treatment facilitates chelation-mediated cadmium detoxification in perennial ryegrass (Lolium perenne L.). Journal of hazardous Materials 389:121849

    doi: 10.1016/j.jhazmat.2019.121849

    CrossRef   Google Scholar

    [86]

    Gururani MA, Ganesan M, Song IJ, Han Y, Kim JI, et al. 2016. Transgenic turfgrasses expressing hyperactive ser599Ala phytochrome a mutant exhibit abiotic stress tolerance. Journal of Plant Growth Regulation 35:11−21

    doi: 10.1007/s00344-015-9502-0

    CrossRef   Google Scholar

  • Cite this article

    Jiang J, Guo Z, Sun X, Jiang Y, Xie F, et al. 2023. Role of proline in regulating turfgrass tolerance to abiotic stress. Grass Research 3:2 doi: 10.48130/GR-2023-0002
    Jiang J, Guo Z, Sun X, Jiang Y, Xie F, et al. 2023. Role of proline in regulating turfgrass tolerance to abiotic stress. Grass Research 3:2 doi: 10.48130/GR-2023-0002

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REVIEW   Open Access    

Role of proline in regulating turfgrass tolerance to abiotic stress

Grass Research  3 Article number: 2  (2023)  |  Cite this article

Abstract: Turfgrasses used on golf courses, sports fields and in urban landscapes have significant ecological and economic value. Various abiotic stresses are crucial in limiting the growth and development of turfgrass. As an important amino acid metabolite, proline can act as a signal to trigger plant responses to stress. Proline accumulation is not only a stress signal, but also alleviates plant damage by maintaining photosynthesis and the activities of antioxidant enzymes and the levels of non-enzymatic antioxidant compounds, thus reducing reactive oxygen species content, and regulating osmosis. A better understanding of mechanisms of proline response to abiotic stress in turfgrass is vital for the development of stress-tolerant germplasm. This review summarizes research progress into the role of proline in regulating growth and physiological and molecular adaptations to abiotic stress, with emphasis on drought, salt and temperature tolerance.

    • Turfgrass is an economically, environmentally, recreationally, and aesthetically important part of urban landscapes[1]. With the demand of rapid urbanization, turfgrass coverage has increased worldwide[2]. Grown in diverse climates and environments, turfgrass often suffers from various abiotic stresses such as heat, cold, drought, and salinity, resulting in the decline of grass quality, growth and development, and other functional attributes[3,4]. Therefore, improvement of turfgrass stress tolerance has been a crucial concern, and a better understanding of mechanisms of turfgrass stress tolerance is of great significance for developing stress tolerant germplasm through breeding and biotechnology.

      Proline is a multifunctional amino acid. It is highly accumulated in plants under various stress conditions, which can confer stress tolerance[5,6]. Proline often exists widely in plants in a free state. As a signal, it plays a key role in regulating gene expression and some metabolic processes[7]. There are two ways for proline biosynthesis to occur in plants: the glutamate pathway or the arginine pathway[7]. As shown in Fig. 1, glutamate (Glu) is reduced to glutamate semialdehyde (GSA) by the bifunctional enzyme Δ1-pyrroline-carboxylate synthetase (P5CS), then GSA is converted to pyrroline-5-carboxylate (P5C), and P5C is further reduced to proline by P5C reductase (P5CR). Ornithine (Orn) produces GSA through ornithine δ-aminotransferase (OAT).

      Figure 1. 

      Proline metabolic pathways in plants: the glutamate pathway. GSA, glutamic-γ-semialdehyde; P5C, Δ1-pyrroline-5-carboxylate; P5CS, Δ1-pyrroline-5-carboxylate synthetase; OAT, ornithine aminotransferase; P5CR, Δ1-pyrroline-5-carboxylate reductase; P5CDH. pyrroline-5-carboxylate dehydrogenase; ProDH, proline dehydrogenase.

      To date, proline accumulation and expression of genes for proline metabolism have been documented in some turfgrass species in response to stress conditions[811]. However, there is no review on the current research status of proline associated with turfgrass stress tolerance. This review summarizes proline research including turfgrass species, types of abiotic stresses, and proline functions. Information provided by this review could be valuable for future studies into the role of proline in regulating turfgrass abiotic tolerance.

    • The role of proline in regulating stress responses has been studied in both cool-season turfgrasses such as Kentucky bluegrass (Poa pratensis), tall fescue (Festuca arundinacea), perennial ryegrass (Lolium perenne), and bentgrass (Agrostis spp.) and warm-season turfgrasses such as bermudagrass (Cynodon dactylon) and zoysiagrass (Zoysia spp.). The keywords TS = (turfgrass* OR lawngrass* OR grass*) AND 'abiotic stress' AND 'proline' was searched. The literature was from the past 10 years from 2013−2022, and the selected language was English. After initial searching, the literature were obtained relevant to proline and abiotic stress in turfgrass. Next, by checking the title and keywords. Finally, reading the full text. From 2013 to 2022, there were close to 100 publications related to proline under abiotic stresses including 22 in bentgrass and 20 in perennial ryegrass, followed by 15 in Kentucky bluegrass, 13 in bermudagrass, 12 in tall fescue, and nine in zoysiagrass (Fig. 2). There were a few publications (ranging from one to five) for other turfgrass species including centipedegrass (Eremochloa ophiuroides), seashore paspalum (Paspalum vaginatum), St. Augustinegrass (Stenotaphrum secundatum) and buffalograss (Bouteloua dactyloides).

      Figure 2. 

      The number of publications in relation to proline in different turfgrass species under stress conditions since 2013. BEN, Bentgrass; PR, Perennial ryegrass; KB, Kentucky bluegrass; BER, Bermudagrass; TF, Tall fescue; ZO, Zoysiagrass; CE, Centipedegrass; SP, Seashore paspalum; AU, St. Augustinegrass; BU, Buffalograss.

      Among the various abiotic stresses, most studies on proline in the past decade were found to deal with drought, salt, cold and heat stresses (Fig. 3). However, there was only one literature report on effects of proline on photoinhibition stress tolerance of turfgrass.

      Figure 3. 

      The number of publications on turfgrasses under different stresses since 2013. The abbreviations on the x- axis from left to right represent drought (DS), salt (SS), cold (CS), heat (HS), waterlogging (WLS), sulfur dioxide (SDS), heavy metal (HMS), and photoinhibition (PS), respectively.

      A network model was created by analyzing keywords in literature extracted from the Web of Science Database in the past 10 years (Fig. 4). The most frequently used keyword was 'lipid peroxidation', and the others were 'freezing tolerance', 'abiotic stress', 'gene expression', 'plant', etc. The lines between keywords in the model showed their correlations, which could indicate the past research hotspots of proline regulation in turfgrass responses to abiotic stresses. It showed that 'lipid peroxidation', 'various abiotic stresses' and 'gene expression' were the most popular keywords for proline related research reports in turfgrass species.

      Figure 4. 

      The analysis of keywords on the research of proline in turfgrass response to abiotic stress (created by authors using CiteSpace). Each circle represents a keyword: the larger the circle, the higher the frequency of the keyword. The lines between the circles represent the relationship between keywords.

    • Proline is considered to have many unique functions in regulating homeostasis in plant tolerance to harsh environments, for example, as an essential amino acid is also a vital osmotic compound to maintain cellular homeostasis in plants[12,13], and as the molecular chaperon it is able to maintain the protein integrity and enhance the activities of different enzymes for preventing oxidative burst in plants by bringing concentrations of reactive oxygen species (ROS) within normal ranges[14]. Numerous studies have reported proline as an antioxidant suggesting its roles as ROS scavenger and singlet oxygen quencher[15,16]. In addition, it has been confirmed that proline is a metal chelator[17,18] and a signaling molecule in plants under adverse stresses[1921]. Although proline metabolism has been studied in turfgrass species[5], the functional roles of proline in regulating turfgrass tolerance to abiotic stresses are not well understood.

    • Drought is a limiting factor for agricultural production worldwide. Proline is the most well-known osmotic protective substance. A large amount of evidence has shown that the accumulation of proline is positively correlated with drought tolerance in plants[4,2225]. Mild drought stress (60% container capacity) induced proline accumulation and improved antioxidant metabolism in creeping bentgrass[26]. The proline, hydrogen peroxide and total ascorbate contents of Agropyron cristatum, A. intermedium, Festuca ovina, Festuca arundinaceae, Cynodon dactylon, Bromus inermis, and B. confinis, sources of low-maintenance turfgrasses for semi-arid regions, increased under drought[27]. Drought stress decreased tall fescue quality, relative water content (RWC), leaf indole-3-acetic acid and cytokinin-zeatin riboside (ZR) contents, and increased proline and abscisic acid (ABA) content, but the tolerant cultivar 'Van Gogh' had greater turfgrass quality rating, RWC, proline, ABA, and ZR content compared to the sensitive 'AST7002' under drought stress (26% container capacity)[28]. Lolium-Festuca complex genotype 'INT-40' showed a higher tolerance to field water deficit and had more denser root growth and more osmotic active compounds accumulated in the shoots, such as proline, trehalose and oligosaccharide[29]. Activities of antioxidant enzymes superoxide dismutase (SOD) and ascorbate peroxidase (APX) increased accompanied with increasing accumulation of proline in tall fescue cultivars 'Pixie' and 'Mini-mustang' under drought stress[30].

      Exogenous application of proline or other molecules that affect proline content may influence turfgrass stress tolerance[3143]. Exogenous application of the spermidine (Spd) improved drought stress tolerance of creeping bentgrass by upregulating proline biosynthesis pathway related genes P5CS and P5CR[44]. Foliar spray of γ-Aminobutyric acid (GABA) and proline alone or in combination improved creeping bentgrass dark green color index and stolon length, but proline treatment alone resulted in higher RWC, indicating that GABA or proline plays a role in creeping bentgrass tolerance to drought stress[31]. Also in creeping bentgrass, plants treated with spermine (Spm) increased nitrogen and proline metabolism, maintained tricarboxylic acid cycle, and enhanced chlorophyll content, photosynthesis, water use efficiency, and cell membrane stability[32]. Exogenous application of plant growth-promoting rhizobacteria isolated from the rhizosphere of Haloxylon ammodendron increased drought tolerance by promoting accumulation of proline, activities of the antioxidant enzymes catalase (CAT) and peroxidase (POD), photosynthetic capacity and RWC of perennial ryegrass[33]. Additionally, the application of exogenous substances such as hydrogen sulfide, abscisic acid, nitrogenous nutrition, silicon or nitric oxide (NO) can further improve the accumulation of proline, associated with high content of chlorophyll, total soluble phenols and glycine betaine in turfgrass species against drought stress[3342]. However, accumulation of proline does not always occur in grass plants under drought stress. For example, exogenous application of 0.5 mM salicylic acid improved zoysiagrass drought tolerance by enhancing the net photosynthetic rate and antioxidant enzyme activities but decreasing proline content and lipid peroxidation compared to the controls[43]. Application of myo-inositol (1 mM) promoted the accumulation of water soluble carbohydrates but decreased drought-induced free proline in leaves of creeping bentgrass, suggesting that the contribution of water soluble carbohydrate to osmotic adjustment under drought stress was greater than that of proline[40].

      SAGIPT41 transgenic bentgrass (IPT encoding isopentenyl transferase) showed better drought tolerance, with higher turfgrass quality, leaf RWC, and OA as well as more soluble proline, sugars, betaine and spermine found in transgenic plants than the control plants[45]. Transgenic tobacco overexpressing proline biosynthesis gene LpP5CS of perennial ryegrass had higher proline content and survival rate after drought treatment[6]. Transcriptome analysis of Kentucky bluegrass showed that two proline dehydrogenase unigenes were down regulated, which could be an advantage to slow the rate of proline degradation after application of ethephon (200 mg·L−1) under drought stress[41]. In brief, the results suggested a role of proline in promoting drought tolerance in turfgrass species.

    • Salt stress is one of the major abiotic stresses in many regions of the world. High salt concentration inhibits plant growth and reduces chlorophyll content, photosystem II photochemical efficiency (Fv/Fm) and K+ content, and increases Na+ accumulation[4648]. The salt tolerant centipedegrass had lower growth inhibition and showed increased proline content and antioxidant enzyme activities than the salt sensitive centipedegrass[46]. When eight C3 turfgrass species were exposed to increasing salt concentration, proline content was positively correlated with salt tolerance, but negatively correlated with salt avoidance, suggesting that variations of salt tolerance among species was caused by the difference in proline content[49]. The proline concentration increased significantly in both a salt-sensitive Kentucky bluegrass and salt-tolerant tall fescue exposed to increasing salt concentration, but tall fescue had less accumulation of Na+ and Cl and higher total soluble sugar than Kentucky bluegrass[48]. The results indicated that accumulating sugars other than proline mainly contributed to salt tolerance of tall fescue. Proline and glycine betaine content also increased with increasing salt concentration in four warm-season turfgrasses including St. Augustinegrass, manila grasses (Zoysia matrella), seashore paspalum and bermudagrass, suggesting a role of proline in cellular protection[47]. A comparative study of salt tolerance of creeping bentgrass (A. stolonifera ) 'Penncross' and rough bentgrass (A. scabra) 'NTAS' showed that the salt tolerant 'NTAS' maintained higher soluble sugar, proline, and glycine betaine accumulations, contributing to higher osmotic adjustment[50].

      The application of some exogenous substances can reduce salt stress injury by increasing proline accumulation. Application of Spd alleviated the reduction of chlorophyll content and K+/Na+ ratio, increased the levels of proline, endogenous Spd, Spm and the activities of ornithine decarboxylase and S-adenosylmethionine decarboxylase and reduced salt injury (200 mM) in Kentucky bluegrass[51]. Exogenous 24-Epibrassinolide (EBR) treatment enhanced the activities of antioxidant enzymes, decreased the content of electrolyte leakage (EL), photosynthetic rate, malondialdehyde (MDA) and hydrogen peroxide (H2O2), and increased the RWC, proline, soluble sugar and soluble protein in leaves of perennial ryegrass under salt stress[52,53]. These results indicated that EBR could improve salt tolerance of perennial ryegrass by enhancing osmotic regulation and antioxidant defense system[52,53]. Foliar spray of GABA alleviated the growth inhibition, increased proline concentration, reduced Na+/ K+ ratio, and significantly increased POD and SOD activities of perennial ryegrass[54]. Foliar application of Si maintained leaf chlorophyll and RWC content, increased shoot length and shoot number, reduced Na+ concentration, but decreased proline content of tall fescue, perennial ryegrass and bermudagrass at all salinity levels, suggesting that other mechanisms than proline accumulation play a role in osmotic regulation[55].

      Under salt stress (255 mM NaCl), the proline biosynthesis gene PrP5CS1 encoding pyrroline-5-carboxylate synthetase was significantly induced in perennial ryegrass leaves, and the up-regulated level of PrP5CS1 in the salt-tolerant cultivar 'Overdrive' was higher than that in sensitive cultivar 'Pizzazz'; at the same time, PrP5CS2 was significantly induced in 'Overdrive' but inhibited in 'Pizzazz'[56]. Transgenic tobacco over-expressing the proline-biosynthesis gene LpP5CS (encoding pyrroline-5-carboxylate synthetase) exhibited higher salt tolerance than the control[6]. The results indicate that salt tolerance of perennial ryegrass may be directly and positively correlated with proline metabolism, and that LpP5CS could be a candidate gene for genetic improvement of salt tolerance. The proline biosynthesis gene LpP5CS were up-regulated after treatment with salt (200 mM NaCl) in roots, stems and leaves of perennial ryegrass[6]. The drought and salt tolerance gene (DST) encoding a C2H2 zinc finger transcription factor negatively regulated salt tolerance in rice (Oryza sativa)[57]. Silencing the OsDST gene enhanced salt tolerance of perennial ryegrass, with higher leaf RWC and lower EL, MDA and H2O2 and proline content found in transgenic plants[58]. Collectively, proline plays a role in molecular regulation salt tolerance of turfgrass species.

    • High and low temperatures are among the main environmental factors that influence plant growth, production and distribution. The high temperature range usually refers to the temperature at which plant growth begins to be inhibited, usually 5−10 °C above the ambient level, however, low temperature injury includes chilling stress (temperature above 0 °C) and freezing stress (temperature below 0 °C)[8]. High temperatures decreased chlorophyll content, Fv/Fm, and RWC, while water-soluble carbohydrates, proline, H2O2, MDA, and EL gradually increased in four cultivars of creeping bentgrass[59,60]. Foliar application of GABA, proline, or N significantly increased creeping bentgrass quality, chlorophyll content and Fv/Fm, and inhibited the activity of chlorophyll degrading enzymes to alleviate leaf senescence[60]. In addition, the levels of plant endogenous proline, GABA, glutamic acid and aspartate were significantly higher after exogenous proline (10 mM) application than those of the control[61], suggesting that proline may regulate 3-phosphoglycerate, GABA shunt, oxaloacetate, secondary metabolism, and pyruvate metabolic pathways to enhance heat tolerance of creeping bentgrass[61]. Significant differences in proline accumulation were found among different ecotypes of Kentucky bluegrass, perennial ryegrass and tall fescue under heat stress, but heat resistant varieties had higher proline content than that less resistant varieties, and this was also accompanied by higher growth rate, tiller number, and antioxidant activities[6265]. Transcriptome analysis showed that up-regulation of genes involved in oxidative protection, proline biosynthesis, lipolysis, hemicellulose, and lignin biosynthesis were detected in heat-tolerant rough bentgrass compared to heat-sensitive creeping bentgrass[66]. The results indicate a positive role of proline in heat tolerance of turfgrass species, although proline accumulation was not always increased in plants with strong heat tolerance[67].

      Freezing stress (−8 °C) increased proline content, sugar, and antioxidants in tall fescue[68]. Cold acclimation (5 °C) resulted in increased proline content in both cold tolerant and cold sensitive varieties of perennial ryegrass, compared to a non-acclimated control, while there were significant differences in proline content between cold tolerant and sensitive varieties[69]. Proline accumulated in creeping bentgrass, Kentucky bluegrass, and perennial ryegrass during cold acclimation improved the content of soluble phenols by regulating the pentose phosphate pathway related to proline, thereby enhancing antioxidant activity and the cold tolerance of the three plants[70]. However, the concentration of proline was generally low and similar between annual bluegrass (Poa annua) and creeping bentgrasss species throughout deacclimation (7 °C), but total soluble sugars, mainly high molecular weight (HMW) fructans, accumulated in each species/ecotypes with higher levels measured in creeping bentgrasss[71]. Perennial grasses often decrease capacity of cold tolerance upon deacclimation, and the low level of proline during deacclimation indicated less cold hardiness plants. Furthermore, transgenic zoysiagrass plants, expressing oat phytochrome A (PhyA) or a hyperactive mutant phytochrome A (S599A), showed a marked increase in proline content compared to non-transgenic control lines. The PhyA lines had approximately 42% increased proline accumulation, expressing S599A showed around 88% increased proline levels compared to the non-transgenic control lines when subjected to cold stress (10 °C)[72]. The results showed that increased proline accumulation was strongly associated with cold tolerance. Transcriptomic analyses reveal that proline synthesis pathways and photosynthesis pathways genes were involved in the regulation of cold response in bermudagrass and zoysiagrass[73]. In Kentucky bluegrass and manila grass, 'proline synthesis process' and 'proline transport' pathways were enriched under cold stress, with many genes in the proline synthesis and degradation pathways up-regulated, such as P5CS, P5CR and P5CD indicating that both the biosynthesis and degradation of proline were activated by cold stress[7476]. DREB (dehydration-responsive element binding) is induced by cold stress, and overexpression of zoysiagrass DREB gene in Arabidopsis thaliana moderately increased the levels of proline and soluble sugar, and improved plant tolerance to high and low temperature stress[77]. Overexpression of the DREB-binding factor PpCBF3 from a cold-tolerant Kentucky bluegrass increased plant growth and survival of Arabidopsis thaliana at extremely low temperatures (4 °C) by protecting photosynthetic components and cell membrane structure, up-regulating proline synthesis and inhibiting ROS formation[78]. The results suggest a connection of proline with the expression of key genes in protecting leaf damage under cold stress. The ICE1 gene is a regulator of cold-induced transcriptional changes in Arabidopsis thaliana, and Korean lawngrass (Zoysia japonica) overexpressing AtICE1 showed increased proline level, higher activities of SOD and POD, and decreased MDA content compared with the wild type under cold stress (4 °C)[79]. The associations of proline with improved cold tolerance were also found in transgenic creeping bentgrass plants overexpressing a Picea wilsonii dehydrin gene (PicW) and tobacco plants overexpressing the creeping bentgrass AstEXP1 gene[80,81].

    • In addition to these stresses mentioned above, sulfur dioxide (SO2), light and heavy metal stresses can also cause serious damage to turf plants. SO2 is the main air pollutant at present. When nine varieties of bermudagrass were exposed to SO2, the SO2 resistant varieties exhibited higher content of soluble sugar, chlorophyll a and proline than that of sensitive varieties, indicating that the tolerance of bermudagrass to SO2 was closely related to proline[82]. Moreover, lower levels of ROS, MDA and EL, and increased antioxidant enzyme activity and proline content were also observed in SO2 tolerant sheep fescue (Festuca ovina) and perennial ryegrass[83].

      Low-light can significantly reduce the quality of the turfgrass, and cause a serious delay in the growth and development of plants[84]. Proline accumulated in tall fescue leaves and roots under low light, and the content of proline was further enhanced by Ca2+ application, suggesting that exogenous Ca2+ promoted the ability of tall fescue to cope with low light stress by restoring various physiological mechanisms including increases in proline content[84].

      The unreasonable discharge of industrial wastewater and waste gas lead to the gradual accumulation of heavy metals such as Cu and Cd in the soil. In perennial ryegrass, Cd stress increased the level of lipid peroxidation and triggered the activity of antioxidant enzymes, and the input of exogenous phosphorus increased the levels of proline and cysteine, partially alleviating the effect of lipid peroxidation, while the absorption of Cd was decreased by increasing phytochelatins[85]. Under heavy metal stress, transgenic zoysia and bentgrass plants carrying S599A-PhyA (oat phytochrome A) showed increased aboveground and underground biomass, antioxidant enzyme activities, chlorophyll and proline contents, and decreased H2O2 levels compared with the control[86]. The results proved a connection between proline and heavy metal tolerance. Overall, as an important metabolite, proline plays multifunctional roles in facilitating turfgrass tolerance to various abiotic stresses.

    • Research into turfgrass has shown that proline accumulation plays highly beneficial roles in promoting turfgrass stress tolerance. The mechanisms of proline for enhancing turfgrass stress tolerance mainly involve osmotolerance for maintaining cellular homeostasis, preventing oxidative injury, and acting as a signaling molecule in gene expression. Much research is still required for a deeper and complete understanding of the functions of proline in response to abiotic stresses in both cool-season and warm-season turfgrasses. The complex morph-physiological and molecular network associated with proline accumulation should be explored more extensively for further genetic engineering of proline metabolism in turfgrasses aimed at improving abiotic stress tolerance.

      • This research was funded by National Natural Science Foundation of China (No. 31971772; 32001407), China Postdoctoral Science Foundation (2022MD723773) and Heilongjiang Postdoctoral Fund (LBH-Z21009).

      • The authors declare that they have no conflict of interest.

      • Copyright: © 2023 by the author(s). Published by Maximum Academic Press, Fayetteville, GA. This article is an open access article distributed under Creative Commons Attribution License (CC BY 4.0), visit https://creativecommons.org/licenses/by/4.0/.
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    Jiang J, Guo Z, Sun X, Jiang Y, Xie F, et al. 2023. Role of proline in regulating turfgrass tolerance to abiotic stress. Grass Research 3:2 doi: 10.48130/GR-2023-0002
    Jiang J, Guo Z, Sun X, Jiang Y, Xie F, et al. 2023. Role of proline in regulating turfgrass tolerance to abiotic stress. Grass Research 3:2 doi: 10.48130/GR-2023-0002

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